Cancer Genome Institute »About CGI »Research Laboratory

The Research Laboratory of the Cancer Genome Institute is at the forefront of cancer genomics, employing state-of-the-art technology to provide comprehensive genetic sequencing services.

Sequencing Services

Investigators requiring sequencing services for research experiments can utilize the expertise of the CGI Research Laboratory, which provides a wide variety of next-generation sequencing services including but not limited to:

• Whole genome sequencing
• Whole exome sequencing
• Whole transcriptome analysis (RNA-Seq)
• High-throughput targeted resequencing
• Epigenetic analysis, including digital restriction enzyme analysis of methylation (DREAM)

Technology

The Laboratory uses the Illumina HiSeq 2000, a powerful next-generation sequencing instrument that provides high sequencing output and fast data generation. In a single run, the machine is capable of sequencing more than 5 human genomes at about 30 times coverage simultaneously, up to 192 gene expression samples, or 100 exome samples.

Features:

• 100-Bp paired end reads
• 2 billion reads per run
• 600-Gb raw sequence data per two-flowcell run
• 200-fold human genomes per run

Expertise

Our staff has expertise in all technical aspects of the aforementioned services using the Illumina platform. Our Biostatistics and Bioinformatics Facility, which has a combined 125 years of experience at Fox Chase, supports the analysis of sequencing data with a 70 node high-performance computing cluster and scalable data storage.

Publications

• CD45-deficient severe combined immunodeficiency caused by uniparental disomy
• Co-authors include Cancer Genome Institute investigators Biao Luo and Suraj Peri.
• Germline BAP1 mutations predispose to malignant mesothelioma
• Contains Cancer Genome Institute-generated data

Contact

For information on sequencing, contact:
Biao Luo, PhD
Director of Research and Development, Cancer Genome Institute
Biao.Luo@fccc.edu
215-728-5677

For information on bioinformatics, contact:
Eric Ross, PhD
Director, Biostatistics and Bioinformatics Facility
Eric.Ross@fccc.edu
215-728-2891