Research at Fox Chase »Research Facilities »Biotechnology

Richard R. Hardy, Ph.D. Senior Member, Director Anita Cywinski, B.A. Technical Specialist

Reimann Building, Room R382 215-728-2445 Richard.Hardy@fccc.edu

Function

The DNA Sequencing Facility provides Fox Chase investigators with computer-readable sequences of their DNA templates in a timely and cost effective manner.

Description

The facility processes samples on an ABI 3130XL capillary genetic analyzer. The facility performs taq cycle sequencing reactions on user-supplied templates and routinely generates sequences of 700-800 bases, with an average of 1-2% ambiguities. Up to two 96 well plates can be loaded and processed automatically on the 3130. Short PCR product sequencing runs can be done during the day, with most sequencing runs processed overnight. The facility provides sequencing primers for all routine vectors. Investigators are provided with a printout of their sequence, including electropherogram traces. Additionally, sequence files are available from a networked file server so that investigators can re-analyze their data on local computers in their laboratories and import the sequence data into software of their choosing for further analysis. All sequence analysis samples are processed using a plate-based setup, providing rapid sample throughput.