DNA Sequencing Facility
- Anita Cywinski, BA, Technical Specialist
The DNA Sequencing Facility (DSF) provides Fox Chase Cancer Center (FCCC) investigators with computer-readable sequences of their DNA templates in a timely and cost-effective manner. The Facility utilizes an ABI 3130xl capillary genetic analyzer and performs taq cycle sequencing reactions in 96-well plates with user-supplied templates, routinely generating sequences of 700-800 bases, with an average of 1-2% ambiguities. Up to two 96-well plates can be loaded on the 3130 for an overnight run. The Facility provides sequencing primers for all routine vectors. Investigators are provided with a printout of their sequence, including electropherogram traces. Additionally, sequence files are available from a networked file server so that investigators can re-analyze their data on local computers in their laboratories and import the sequence data into software of their choosing for further analysis. The Facility routinely processes over 1,000 samples per month.