Cell Sorting Facility
- Jim Oesterling, BS, Scientific Technician I
The Cell Sorting Facility (CSF) offers Fox Chase Cancer Center (FCCC) investigators the capability of analyzing cells by 14 independent measurements, 12 of which are based on fluorescence and 2 on light scatter. Major applications of the flow cytometer include discrimination and isolation (sorting) of lymphocyte subpopulations (at up to 20,000 cells/sec), isolation of rare variant (1/107) cell lines, characterization of tumors or cell lines, and single cell cloning of cell lines by direct deposition into 96-well plates. In addition, flow cytometers in the Facility can be used to measure DNA content (cell cycle) and calcium flux upon cell activation. The Facility also aids users with advice on experimental design (including availability of reagents and appropriate fluorochromes), sample preparation and data interpretation.