Laser Capture Microdissection Services
Laser capture microdissection (LCM)
Instrumentation and expertise needed for the isolation of pure populations of cells from heterogeneous tissue specimens. LCM services include identification and isolation of tumor cells or other cells of interest, as well as DNA, RNA and protein purification from LCM samples. This Core is equipped with two LCM systems (PixCell II® and Veritas™, Molecular Devices), which are available for a wide range of applications, including LCM of specific immunostained or fluorescent cells. A SYBR Green assay is utilized to screen formalin-fixed paraffin-embedded (FFPE) tissue blocks for intact RNA that is suitable for microarray or RT-PCR analysis. Amplification of limited quantities of total RNA from microdissected frozen and FFPE tissues is also available.