Cell Biology Facilities
The facilities and resources have been structured to fulfill the needs of the multi-disciplinary research programs at Fox Chase Cancer Center. The facilities and resources have been designed to enhance on-going research by supplying information, reagents, and technical expertise that are not readily available to the individual investigator.
Biological Imaging Facilities
The Biological Imaging Facilities provide three separate services to users who are interested in:
- This is a hands-on facility that allows users to examine specimens with high resolution fluorescence, phase and DIC microscopy. Our equipment includes high resolution epifluorescence as well as a multi-wavelength spectral confocal microscopes.
- This Facility provides users with ultrastructural analysis of biological samples ranging from purified nucleic acids and proteins to subcellular organelles, whole cells and organs or organisms. Due to the highly specialized nature of the sample preparation for EM, it usually functions as a full service facility.
- The Small Animal Imaging component of the bio-imaging facility gives investigators access to systems for magnetic resonance imaging(MRI) and bioluminescence imaging, and endoscopic examinations. MRI provides users with the capability of non-invasive measurements of tumor volume, while more sophisticated experiments involve measurements of tumor perfusion based on contrast agent kinetics. Bioluminescence imaging (BLI) is performed on tumor bearing animals that have been transfected with luciferase (firefly or renilla) or a fluorescent protein. Up to five mice can be imaged simultaneously in our BLI system in order to detect tumors and follow tumor growth with high throughput. Finally, tumors in the mouse colon can be imaged directly with a unique endoscopy system.
All three facilities provide state-of-the-art technologies as well as technical expertise to assist end users with diverse research needs.More
Cell Culture Facility
The Cell Culture Facility provides reagents, equipment, technical expertise, consultation, and training to support mammalian cell culture efforts at FCCC. Our services include basic cell culture support, cell propagation, generation of culture supernatants, hybridoma production to generate monoclonal antibodies, establishing gene-manipulated mouse embryonic stem cells, expertise in establishing primary cultures from human and animal tissue samples, cell transformation, cell banking, screening for detecting mycoplasma contamination in cultured cells, consultation to assist in experimental design, and providing sterile workstations for use by investigators who do not have facilities of their own.More
Cell Sorting Facility
The Cell Sorting Facility (CSF) offers Fox Chase Cancer Center (FCCC) investigators the capability of analyzing cells by 14 independent measurements, 12 of which are based on fluorescence and 2 on light scatter.More
The Glasswashing Facility collects, washes, dries, and sterilizes glassware owned by individual laboratories at Fox Chase. The service is provided according to a prearranged schedule based upon each laboratory's needs. The glassware is returned the same day if it is picked up before noon. In the event of a critical need for glasswashing services, the facility is capable of processing an investigator's request.More
The Histopathology Facility's pathologists and technicians provide Cancer Center members with technical processing of cells and tissues and interpretation of microscopic results.More