- Emmanuelle Nicolas, PhD
215-728-2489 (Manager); 215-214-1696 (Staff)
- CPP P3013
Genotyping and Real-Time PCR performs PCR-based services including genotyping for investigators who maintain colonies of transgenic and knock-out mice in their research, mutations and epigenetic alterations analyses and quantification of nucleic acids. New assays are developed based on investigators' needs. All primers and probes for in-house developed assays are kept in a database ready for sharing by all the users.
Genotyping and Real-Time PCR occupies approximately 600 square feet on the second floor of the Cancer Prevention Pavilion. Laboratory equipment includes the PSQ96MA (Pyrosequencing), CEQ8800 (fragment analysis), two ABI7900 (Real-Time PCR), a Corbett Robotics PCR pipetting robot, an Agilent Bioanalyzer (lab-on-a-chip), a Nanodrop spectrophotometer, an Eppendorf MasterCycler Gradient Thermal Cycler, an MJ Research PTC-225 Tetrad Thermal Cycler and a BioRad Dyad Thermal Cycler. A Baker SterilGuard II hood is dedicated exclusively to the preparation of PCR reactions. An Eppendorf 5075VAC System is used for automated DNA extractions from tissues and cells. General laboratory equipment includes horizontal electrophoresis apparatus for DNA analysis, a Corning pH meter (model 340), refrigerator and freezers (-20°C and -80°C) for reagent and specimen storage, non-circulating water bath, and dry block incubators. Equipment rooms are located across the hall and provide access to a UV box and an Alpha Imaging System for gel documentation and a Baker SterilGuard II hood dedicated to biohazard sample handling.