FEBS Advanced Course
Yeast two-hybrid systems:
powerful tools for analysis of protein-protein interactions
Moscow, October 28 - November 2, 2002
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Course Description |
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Aim of the course
The original two-hybrid system (YTH), an experimental approach designed to detect protein interactions, exploited the modular nature of many transcription factors. It has provided the intellectual and technical seed for the evolution of an array of innovative approaches, the application of which broaden the scope of experimentally addressable questions bearing on the interaction of proteins with a diverse group of partner molecules. The available array of modified and alternative approaches facilitates the analysis of complex cellular machinery and signaling networks that rely on multiple interactions which involve protein components. Such advances have facilitated the functional analysis of proteins on the genome level, a feat considered untenable a decade ago. At the same time, YTH remains a relatively simple, quick and low cost technique, when compared to alternative approaches used to define interacting groups based on physical interactions (eg., co-purification of complexes of interest followed by use of mass spectrophotometry to assign identity of individual protein components). Scientific content of the course will give both the theoretical background and the practical, hands-on experience to the participants. It is expected that upon completion of this class, a participant would be able to set up a “basic” two-hybrid screen and to use YTH to detect interaction between defined proteins without a need in additional sources of information. We would also provide limited information on other, more specialized YTH-based techniques, so that participants could choose the most appropriate technique for their particular field of study. Other activities:
Number of participants is limited to 20 in order to maximise the effectiveness of hands-on experience in YTH methodology. The participants will be chosen based on the basis of their academic and/or research backgrounds, taking into account the relevance of the course to their needs and career development. No poster session is planned, but participants are encouraged to bring their recent poster presentations. The course is organized for young researchers from the Former Soviet Union and Eastern Europe. Registration fee: 250 Euro. Deadline for applications: September 1, 2002. |
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Geography of Applications |
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This map shows the locations of the research institutions, from which we have received applications.
So can you guess the names of the countries at least? What about cities? Hint:Mouse over the dots to get the names of Institutions, from where the applications were received
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two-hybrid
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