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Two-hybrid kit request form
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Finally, you can submit your
project here and request YTH plasmids!
Please read carefully:
Depending on your choice, we are going
to send you plasmids to clone your protein(s) of interest. If you intend
to do a standard library screen, you will receive a LexA-fusion bait plasmid
pMW103. If you plan to use Dual Bait, you will receive in
addition the cI-fusion bait plasmid pGBS10. Finally, if you just need to test interaction between
defined pais of proteins, we will send you pMW103and pJG4-5 (an activation domain
fusion plasmids).
Prior to use in a two-hybrid application, your baits (and preys) have to be
characterized. If you clone your genes of interest into the plasmids we send
you, we will characterize your constructs right on our course, in parallel
with control constructs. We will test them for activation and expression (on
Western blot), using the antibody to LexA and cI. That would help you understand
whether your baits are usable or require modification...
We will only do this if more than a half of participants
prepare their clones.
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