| 1 |
Introduction to the yeast
two-hybrid system; Two-hybrid system components. The interaction-trap
system as an example |
| 2 |
Yeast genetics |
| 3 |
Bait Assessment |
| 4 |
Characterization of primary
positives. Interaction mating |
| 5 |
Proteomics and the two-hybrid
system |
| 6 |
Variations & New Developments.
Commercially available systems: LexA- & Gal4-based systems |
| 7 |
Using YTH in pharmaceutical
screening |
| 8 |
Two-hybrid: what are your chances
on success? Two-hybrid on the Internet |
| 9 |
False Positives |
| 10 |
Dual Bait system |
| 11 |
Split-ubiquitin system and
other non- trancription-based systems |
| 12 |
Bacterial two-hybrid systems |
| 13 |
Bioinformatics: extending
your clone in silico |
| 14 |
Using YTH to study interactions
between components of multisubunit RNA polymerases |