Usage of the IT libraries

(for the first hundred of analyzed screenings)

1. Libraries listed in Current Protocols

were used in 79 screenings

# Source of RNA/DNA; link to request: Indepen-
dent Clones		 Insert Size (Avg.) Usage/success

(+, -, under investigation)

 Constructed by/ link to the original article
1 HeLa cells (human cervical carcinoma), exponentially growing, cDNA 
        6
9.6x10
 0.5-2.0 kb (1.0 kb) IIIIIIIIIIIIIIIIII
IIIIIIIIII
IIIIIII
total 35; 18+, 7 -, 10?		 J. Gyuris
2 WI-38 cells (human lung fibroblasts), serum starved, cDNA 
        6
5.7x10
 0.3-3.2 kb (1.4 kb) II
II
total 3: 2+, 1?		 C. Sardet and J. Gyuris
3 Jurkat cells (human T cell leukemia), exponentially growing, cDNA 
       6
4.0x10
 0.7-2.8 kb (1.5 kb) I
II
total 2: 1+, 1?		 W. Kolanus, J. Gyuris, B.Seed
4 fetal brain, 22 weeks, human, cDNA 
        6
3.5x10
 (1.5 kb) IIIIIIIIIIIII
III
I
total 17: 13+; 1-, 3?		 D. Krainc
5 liver, human, cDNA 
     6
> 10
 0.6-4.0 kb (>1 kb) ? J. Pugh
6 CD4+ T cell, murine, cDNA 
     6
> 10
 0.3-2.5 kb (>0.5 kb) II

total 2; 2+		 V. Prasad
7 CHO cells (Chinese Hamster Ovary), exponentially growing, cDNA 
        6
1.5x10
 0.3-3.5 kb (1 kb) ? V. Prasad
8 Drosophila melanogaster, 0-12 hour embryos, cDNA 
       6
4.2x10
 0.5-2.5 kb (1.0 kb) I
II
I
total 4; 1+, 1-, 2?		 R. Finley
9 Drosophila melanogaster, ovary, cDNA 
        6
3.2x10
 0.3-1.5 kb (800 bp) IIII
I
I
total 6; 4+, 1-, 1? 		R. Finley
10 Drosophila melanogaster, disc, cDNA 
       6
4.0x10
 0.3-2.1 kb (900 bp) I
III
total 4; 1+, 3?		 R. Finley
11 S. cerevisiae, S288C, genomic 
         6
> 3 x10
 0.8-4.0 kb IIII
I
I
6:total 4+, 1-, 1? 		P. Watt
Color code: red: success; blue: failure ; green: ongoing


2. Recently constructed libraries

were used in 22 screenings

# Source of RNA/DNA Indepen- dent Clones Insert Size (Avg.) Usage/success Constructed by/ contact to:
1 day 18 rat brain cDNA library . . II
 I
total 3; 2+, 1-		 .
2 HeLa adenovirus infected . . I
total 1; 1+		 .
3 human 293 adenovirus infected (early and late stages) . . I
total 1; 1+		 .
4 mouse embrio . . I
II
total 3; 1+, 2?		 .
5 mouse pachyten spermatocytes . . II
total 2; 2?		 .
6 G0 fibroblast . . I
total 1; 1+		 .
7 MDCK polyA+ . . I
total 1; 1+		 .
8 HepG2 cell line cDNA . . I
total 1; 1+		 .
9 Trypanosoma brucei . . I
total 1; 1+		 .
10 Agrobacterium tumefaciens . . I
total 1; 1?		 .
11 Drosophila melanogaster, head . . I
 I
total 2; 1+, 1?		 .
12 Arabidopsis thaliana, 7 day old seedlings . . I
 I
total 2; 1+, 1-		 .
13 tomato/P.syringae 8 x 106 . II
I
total 3; 2+, 1?		 G.Martin

2 colleagues did not give the names of the RFLY libraries they used; finally, some investigators used the libraries designed for the other systems and selected the interators using only lacZ screen.

http://www.clontech.com/clontech/JUL96UPD/JUL96MMLibraries.html

Back to the introduction page

Click here to see the table of analyzed library screenings

See alist of articles using any kind of two-hybrid system (published till October 1995)

Go to the Table summarizing some of the two-hybrid system usage

Request the appropiate strains and plasmids in the Roger Brent lab. or fill our questionnaire.