Sources of Interaction Trap Reagents/Manuals

• Introduction to Two-Hybrid
• The Yeast Two-Hybrid System., ed. Paul L. Bartel and Stanley Fields, Oxford University Press, 1997
• See manuals listed below

• Sources of Interaction Trap compatible libraries

• Sources of Interaction Trap reagents (detailed description)

• Sources of commercially available reagents

• Recent Manuals on Interaction Trap:
• Golemis, E., Serebriiskii, I., Gyuris J. and Brent, R. Interaction Trap/ Two-Hybrid Systems to Identify Interacting Proteins. Unit 20.1

In: Current Protocols in Molecular Biology, ed. Ausubel, F.M., et al., John Wiley & Sons, NY, 1997.
Updated and reprinted in:
• Current Protocols in Protein Science, 1998
• Current Protocols in Neuroscience, 1999
• Golemis, E.A. and Serebriiskii, I. "Two-hybrid approaches to analysis of protein-protein interaction", in Cells: a Laboratory Manual, eds. D.L. Spector, R. Goldman, and L. Leinwand, Cold Spring Harbor Laboratory Press, 1997.
• Golemis, E.A., Serebriiskii, I., and Law, S. Adjustment of Parameters in the yeast two-hybrid system: criteria for detecting physiologically significant protein-protein interactions", p. 11-28. In: Current Innovations in Molecular Biology: Gene Cloning and Analysis, ed. B. Schaefer, Horizon Scientific Press, 1997.

• On-line manuals & protocols
• Invitrogen Manuals: download pdf files
• Hybrid Hunter Kit
• Hybrid Hunter Library Support Guide
• Clontech Manuals: download pdf file
• MATCHMAKER LexA Two-Hybrid System,
• also see Addendum
• Also, look in Clontechniques July 96
• Origene's manual: download pdf file
• Interaction mating protocol (Finley Lab)
• 2 Hybrid System TRAFO Protocol (just transformation)

 
• Reviews / protocol books coming in year 2000
• Golemis, E.A. and Serebriiskii, I. Recent developments in two hybrid technology. In the 3rd edition of Molecular Cloning: a Laboratory Manual, ed. J. Sambrook. Cold Spring Harbor Laboratory Press, 2000 (the former Maniatis Cloning manual). Includes both review and a protocol.
• " Methods in Molecular Biology:  Two Hybrid Systems, Methods, and Protocols", ed. P.MacDonald. Humana Press
• Serebriiskii, I., G.Toby, R.L. Finley, and E.A.Golemis. Genomic analysis utilizing the yeast two-hybrid system. In: " Methods in Molecular Biology: Genomic Protocols", ed. M. Starkey. Humana Press
• Fashena, S. J.,  Serebriiskii, I., and Golemis, E.A. LexA based two hybrid systems. In "Methods in Enzymology: Chimeric Genes and Proteins", ed. J.Abelson, M.Simon, S.Emr, J.Thorner. Academic Press
• Serebriiskii, I., Mitina, O.,  Chernoff, J. , and E.A.Golemis. Use of a two-hybrid dual bait system to discriminate specificity of protein interactions in small GTPases. In "Methods in Enzymology: Ras Regulators and Effectors", ed. C.J. Der. Academic Press