DupLEX-A™ Yeast Two-Hybrid System
Hybrid Hunter™ Yeast Two-Hybrid System
Matchmaker LexA Yeast Two-Hybrid System
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DupLEX-A™ Yeast Two-Hybrid System |
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Hybrid Hunter™ Yeast Two-Hybrid System |
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Matchmaker LexA Yeast Two-Hybrid System |
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DisplayGREEN Two-Hybrid Kit System |
At present, two Interaction Trap compatible systems are commercially available. Clontech's Matchmaker LexA two-hybrid system, as well as Origene's DupLex, uses a basic set of plasmids, while Invitrogen has developed its own bait and library plasmids. Both systems use the most sensitive reporters (EGY48 and pSH18-34), and provide their own positive and negative controls for testing activation or interaction between defined proteins. Also, see the detailed description page .
pHybLex/Zeo, bait plasmid constructed by Invitrogen, is significantly smaller (half the size of the original pEG202), and has an enriched polylinker. For plasmid selection, it utilises a gene that confers resistance to the antibiotic Zeocin (supplied with the kit), expressed in both bacteria and yeast. The Zeocin resistance gene eliminates auxotrophic selection for the bait plasmid (so either HIS3 or TRP1 two-hybrid libraries could be in principle used in combination with this bait plasmid). Additional advantages, including bypass of KC8 in recovery of library plasmid to bacteria and potential use in tri-hybrid applications, are detailed by Invitrogen. Working with Zeocin, however, can be a pain.
Display Systems Biotech uses the same bait and prey plasmids, as well as selection strains, as the original Interaction Trap; oldfashioned lacZ reporter was, however, replaced with fancy GFP reporter.
Premade libraries available from these companies are listed on Libraries page, and custom made libraries are also available upon request.
* Disclaimer
We note, we gain no commercial benefit (unfortunately!) from description
of these reagents.