Libraries compatible with the IT system

Last updated: 16 Feb. 2000

Source of RNA/DNA Vect. Indep. Clones, x 106 Insert Size, kb (Avg.) Contact information
    Human I 
HeLa cells (human cervical carcinoma) JG 9.6 0.3-3.5 (1.5 ) RB, Clontech,
Invitrogen, OriGene, Display
HeLa cells (human cervical carcinoma) Y 3.7 0.3-1.2  Invitrogen
WI-38 cells (human lung fibroblasts), serum starved, cDNA JG 5.7 0.3-3.5 (1.5 ) RB, Clontech,
OriGene, Display
Jurkat cells (human T cell leukemia), exponentially growing, cDNA JG 4.0 0.7-2.8 (1.5 ) RB
Jurkat cells (human T cell leukemia), Y 3.2 0.3-1.2  Invitrogen
Jurkat cells (human T cell leukemia), JG 3.0 0.5-4.0 (1.8 ) Clontech
Jurkat cells (human T cell leukemia) JG 5.7  (>1.3 ) OriGene, Display
Jurkat cells (human T cell leukemia)  JG 2.0  0.7-3.5 (1.2 ) Stephen Witte
Be Wo cells (human fetal placental choriocarcinoma) Y 5.4 0.3-0.8  Invitrogen
Human Lymphocyte JG 4.0 0.4-4.0 (2.0 ) Clontech
Human Lymphocyte, HTLV tr. line SLB1
JG
41
0.2-4.1
 Display
Human B cell lymphoma JG .  .  Huifeng Niu
HeLa, adenovirus infected JG .  .  . 
human 293 adenovirus infected (early and late stages) JG .  .  Kurt Gustin
G0 fibroblast  JG .  .  . 
MDCK cells JG .  .  Dayue Chen 
HepG2 cell line cDNA JG 2 .  Margherita Melegari
MCF7, Breast cancer cells, estrogen-depleted  JG  10 0.6-4.5
(>1.5 )		 OriGene, Display
MCF7, Breast cancer cells, estrogen-treated  JG 10 0.4-4.6
(>1.1 )		 OriGene, Display
MCF7 cells, serum-grown
JG
10
0.4-3.5
 OriGene, Display
MG63 cells, osteosarcoma cell line
JG
3.0
0.3-2.5
 . 
Breast
Y
9
 . Invitrogen
Breast tumor
Y
8.84
 . Invitrogen
Lung 
Y
5.9
 . Invitrogen
Lung Tumor
Y
1.9 
 . Invitrogen
Human Liver JG  1 0.6-4.0 (>1 ) RB
Human Liver JG 11  (>1 ) OriGene, Display
Human Liver JG 2.2 0.5 - 4 (1.3) Clontech
Human Liver Y 3.2 0.3 - 1.2  Invitrogen
Human Fetal Liver
JG
8.5
0.3 - 2.4
 MoBiTec, Display
Human Brain JG 3.5 0.5 - 4.5 (1.4) Clontech
Human Brain Y 8.9 0.3 - 1.2  Invitrogen
Human Brain
JG
3.7
0.3 - 4.7
 MoBiTec, Display
Human Testis Y 6.4 0.3 - 1.2  Invitrogen
Human Testis JG 3.5 0.4 - 4.5 (1.6) Clontech
Human Testis
JG
3.1
0.3 - 4.5
 MoBiTec, Display
Human Ovary Y 4.6 0.3 - 1.2  Invitrogen
Human Ovary JG 4.6 0.4-4.6
(>1.3 )		 OriGene, Display
Human Ovary
JG
3.5
0.5 -4.0
(1.8)
 Clontech
SKOV3, Ovarian Cancer Y 5.0 0.2-2.3
(>1.4 )		 OriGene, Display
SR3 cell
JG
5.4
0.5 -4.0
 . 
Human Heart JG 3.0 0.3 - 3.5 (1.3) Clontech
Human Heart
JG
3.1
0.3 - 3.7
 MoBiTec, Display
Human Placenta Y 4.8 0.3 - 1.2  Invitrogen
Human Placenta JG 9.6 0.3 - 4.0 (1.2) Clontech
Human Placenta
JG
9.1
0.3 - 4.5
 MoBiTec, Display
Human Peripheral Blood Leukocyte JG 10  (>1.3 ) OriGene
Human Mammary Gland JG 3.5 0.5 - 5 (1.6) Clontech
Human Mammary Gland
JG
4.1
0.3 - 4.5
 Display
Human Kidney JG 3.5 0.4 - 4.5 (1.6) Clontech
Human Kidney
JG
3.4
0.3 - 4.1
 Display
Human Fetal Kidney JG 3.0 0.2-2.0 (>1 ) OriGene, Display
Spleen
Y
11.4
 .  Invitrogen
Prostate
Y
5.5
 .  Invitrogen
Human Normal Prostate JG 1.4 0.4-4.5 (1.7 ) Clontech
Human Normal Prostate JG 6.4 0.3-1.7
(>1 )		 OriGene, Display
Human Prostate Cancer JG 4.8 0.2-2.0
(>1.1 )		 OriGene, Display
LNCAP Prostate cell line, untreated JG 2.9 0.3-2.0
(>0.8 )		 OriGene, Display
LNCAP Prostate cell line, Androgen treated JG 4.6 0.3-2.3
(>0.9 )		 OriGene, Display
Human Fetal Prostate JG .  .  OriGene
Human Fetal Liver JG 3.5 0.3 - 4.5 (1.3) Clontech
Human Fetal Liver JG 8.6 0.4 - 2.5 OriGene, Display
Human Fetal Liver Y 2.37 0.3 - 1.2  Invitrogen
Human Fetal Brain JG 3.5 0.5-1.2 (1.5) RB, Clontech,
Invitrogen, OriGene, Display
    Rodents I 
CD4+ T cell, murine, cDNA JG > 1 0.3-2.5 (>0.5 ) RB
CHO cells (Chinese Hamster Ovary), exponentially growing, cDNA JG 1.5 0.3-3.5 (1) RB
A20 cells (Mouse B cell lymphoma) Y 3.11 0.3 - 1.2  Invitrogen
Mouse pachytene spermatocytes JG .  .  Christer Hoog
Mouse Brain JG 6.1 0.3-1.7
(>1 )		 OriGene, Display
Mouse Brain JG 4.5 c 4.5 (1.2) Clontech
Mouse Breast, lactating
JG
10
0.4-3.1
 OriGene
Mouse Breast, involuting
JG
10
0.4-7.0
 OriGene
Mouse Breast, virgin
JG
10
0.4-5.5
 OriGene, Display
Mouse Breast, pregnant (12 days)
JG
6.3
0.4-5.3
 OriGene
Rat Adipocyte, 9 week old Zucker rat
JG
10
0.4-5.0
 OriGene
Rat Brain  JG
4.5
0.3 - 3.4 
 OriGene, Display
Rat Testis JG 8.0 (>1.2 ) OriGene, Display
Rat Thymus JG 8.2 (>1.3 ) OriGene
Mouse Liver JG 9.5 0.3-3.2 (>1.4 ) OriGene, Display
Mouse Skeletal Muscle
JG
7.2
 0.4-3.5 OriGene
Mouse Spleen JG 10  (>1 ) OriGene
Mouse Ovary JG 4.0 0.3-1.5 (>1.2 ) OriGene, Display
Mouse Prostate JG
3.7. 
0.3 - 4.0
 OriGene, Display
Mouse Embryo, whole (19 day) JG
.10
. 0.2-2.5
OriGene, Display
Mouse Embryo JG 3.6 0.5 - 5 (1.7) Clontech
    Fruit fly I 
Drosophila melanogaster, adult, cDNA JG 1.8 0.3-1.8
(>1.0 )		 OriGene, Display
Drosophila melanogaster, embryo, cDNA JG 3.0 0.5-3.0 (1.4) Clontech
Drosophila melanogaster, 0-12 hour embryos, cDNA JG 4.2 0.5-2.5 (1.0 ) RB
Drosophila melanogaster, ovary, cDNA JG 3.2 0.3-1.5 (800 bp) RB
Drosophila melanogaster, disc, cDNA JG 4.0 0.3-2.1 (900 bp) RB
Drosophila melanogaster, head JG .  .  Michael Rosbash
    yeast / bacteria / plants I 
S. cerevisiae, S288C, genomic JG > 3 0.8-4.0  RB
S. cerevisiae, S288C, genomic
JG
4.0
0.5-4.0 
 OriGene, Display
Trypanosoma brucei JG .  .  . 
Agrobacterium tumefaciens JG .  .  . 
Arabidopsis thaliana, 7 day old seedlings  JG .  .  Howard M. Goodman
tomato/P.syringae JG 8 .  Gregory B. Martin
    Diverse I 
MDBK Cell, Bovine Kidney  JG 5.8 0.5-1.7 (>1.2 ) OriGene, Display
MG63 Cell
JG
3.0
0.3-2.5
 OriGene
SKBR3 Cell
JG
5.4
0.5-4.0 
 OriGene
Xenopus laevis Embryo JG 2.2 0.3 - 4 (1.0) Clontech
Xenopus laevis Embryo
JG
2.1
0.2 - 3.9
 MoBiTec, Display
C. elegans JG 3.8 0.2-3.6
(>1.2 )		 OriGene
Sea Urchin Ovary JG  3.5 0.5 - 3.5 
(1.7 )		 Clontech
Synthetic aptamers pJM-1 > 1000 60 bp RB
Abbreviations:
RB: Roger Brent lab.; JG, pJG4-5 plasmid; Y, pYesTrp2 plasmid

Note: some libraries are KNOWN to be the same for all companies (e.g., reamplified highly successful HeLa and Fetal Brain libraries). Some libraries are, however, JUDGED to be the same based on their descriptions (number of clones and average size); this is the case for many libraries sold my Origene, Display Biosystems and MoBiTec. Prices on the same libraries are, however, VERY different. Check with the companies!
Note: if no contact information is given: library exist, and was successfully used at least once; but, so far, the author did not responded (either positive or negative) to our request. It does not make sense to contact us therefore; a better idea would be to post a message in a corresponging newsgroup(s).

For the most up to date information, contact the biotech companies.