Note: PsrI in polylinker are not unique and cannot be used for cloning. Two frames are available.
pGBS10 complete sequence
LOCUS PGBS10B .G 5310 BP DS-DNA CIRCULAR SYN 15-JAN-1998
DEFINITION -
ACCESSION -
KEYWORDS -
SOURCE -
FEATURES Location/Qualifiers
promoter <1..1
/note="ADH1 promoter"
misc_binding 2..33
/note="myc binding site "
gene 61..69
/note="LexA protein - N terminal region"
promoter 70..539
/note="ADH1 promoter"
gene 555..1267
/note="cI gene"
terminator 1348..1540
/note="ADH Terminator"
rep_origin 1675..2832
/note="2µ origin"
frag 3090..4292
/note="71 to 1273 of pFA6a-kanMX4"
frag 3090..4292
/note="1 to 1203 of Kan/G418 (Bgl/Sca)-kanMX4 (C++ cut
with BamH1/StuI)"
misc_binding 3134..3477
/note="TEF promoter (AG)"
ORF 3478..4287
/note="Kan R gene from Tn903"
terminator 4310..4582
/note="cytC terminator"
rep_origin 4617..4886
/note="ori"
frag <5309..>5310
/note="1 to 1768 of HIS5 PCR"
BASE COUNT 1431 A 1187 C 1160 G 1532 T 0 OTHER
ORIGIN -
1 ATGGAACAAA AACTCATCTC AGAAGAGGAT CTGAATCAGC TTGAATTAAT TCCGGGCGGA
61 ATGAAAGCGT TCCATCATCA TATCGAAGTT TCACTACCCT TTTTCCATTT GCCATCTATT
121 GAAGTAATAA TAGGCGCATG CAACTTCTTT TCTTTTTTTT TCTTTTCTCT CTCCCCCGTT
181 GTTGTCTCAC CATATCCGCA ATGACAAAAA AAATGATGGA AGACACTAAA GGAAAAAATT
241 AACGACAAAG ACAGCACCAA CAGATGTCGT TGTTCCAGAG CTGATGAGGG GTATCTTCGA
301 ACACACGAAA CTTTTTCCTT CCTTCATTCA CGCACACTAC TCTCTAATGA GCAACGGTAT
361 ACGGCCTTCC TTCCAGTTAC TTGAATTTGA AATAAAAAAA GTTTGCCGCT TTGCTATCAA
421 GTATAAATAG ACCTGCAATT ATTAATCTTT TGTTTCCTCG TCATTGTTCT CGTTCCCTTT
481 CTTCCTTGTT TCTTTTTCTG CACAATATTT CAAGCTATAC CAAGCATACA ATCAACTCCA
541 AGCTGATCTA CCGTATGAGC ACAAAAAAGA AACCATTAAC ACAAGAGCAG CTTGAGGACG
601 CACGTCGCCT TAAAGCAATT TATGAAAAAA AGAAAAATGA ACTTGGCTTA TCCCAGGAAT
661 CTGTCGCAGA CAAGATGGGG ATGGGGCAGT CAGGCGTTGG TGCTTTATTT AATGGCATCA
721 ATGCATTAAA TGCTTATAAC GCCGCATTGC TTGCAAAAAT TCTCAAAGTT AGCGTTGAAG
781 AATTTAGCCC TTCAATCGCC AGAGAAATCT ACGAGATGTA TGAAGCGGTT AGTATGCAGC
841 CGTCACTTAG AAGTGAGTAT GAGTACCCTG TTTTTTCTCA TGTTCAGGCA GGGATGTTCT
901 CACCTGAGCT TAGAACCTTT ACCAAAGGTG ATGCGGAGAG ATGGGTAAGC ACAACCAAAA
961 AAGCCAGTGA TTCTGCATTC TGGCTTGAGG TTGAAGGTAA TTCCATGACC GCACCAACAG
1021 GCTCCAAGCC AAGCTTTCCT GACGGAATGT TAATTCTCGT TGACCCTGAG CAGGCTGTTG
1081 AGCCAGGTGA TTTCTGCATA GCCAGACTTG GGGGTGATGA GTTTACCTTC AAGAAACTGA
1141 TCAGGGATAG CGGTCAGGTG TTTTTACAAC CACTAAACCC ACAGTACCCA ATGATCCCAT
1201 GCAATGAGAG TTGTTCCGTT GTGGGGAAAG TTATCGCTAG TCAGTGGCCT GAAGAGACGT
1261 TTGGGAATTT GGAATTCGAG CTCAGATCTC AGCTGGGCCC GGTACCGCGG CCGCTCGAGT
1321 CGACCTGCAG CCAAGCTAAT TCCGGGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA
1381 ATAAGTTATA AAAAAAATAA GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG
1441 AAAATTCTTG TTCTTGAGTA ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGCA
1501 TGAGGTCGCT CTTATTGACC ACACCTCTAC CGGCATGCCG AGCAAATGCC TGCAAATCGC
1561 TCCCCATTTC ACCCAATTGT AGATATGCTA ACTCCAGCAA TGAGTTGATG AATCTCGGTG
1621 TGTATTTTAT GTCCTCAGAG GACAATACCT GTTGTAATCC GTCCCAAGCT AACGAAGCAT
1681 CTGTGCTTCA TTTTGTAGAA CAAAAATGCA ACGCGAGAGC GCTAATTTTT CAAACAAAGA
1741 ATCTGAGCTG CATTTTTACA GAACAGAAAT GCAACGCGAA AGCGCTATTT TACCAACGAA
1801 GAATCTGTGC TTCATTTTTG TAAAACAAAA ATGCAACGCG AGAGCGCTAA TTTTTCAAAC
1861 AAAGAATCTG AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAGAGCGC TATTTTACCA
1921 ACAAAGAATC TATACTTCTT TTTTGTTCTA CAAAAATGCA TCCCGAGAGC GCTATTTTTC
1981 TAACAAAGCA TCTTAGATTA CTTTTTTTCT CCTTTGTGCG CTCTATAATG CAGTCTCTTG
2041 ATAACTTTTT GCACTGTAGG TCCGTTAAGG TTAGAAGAAG GCTACTTTGG TGTCTATTTT
2101 CTCTTCCATA AAAAAAGCCT GACTCCACTT CCCGCGTTTA CTGATTACTA GCGAAGCTGC
2161 GGGTGCATTT TTTCAAGATA AAGGCATCCC CGATTATATT CTATACCGAT GTGGATTGCG
2221 CATACTTTGT GAACAGAAAG TGATAGCGTT GATGATTCTT CATTGGTCAG AAAATTATGA
2281 ACGGTTTCTT CTATTTTGTC TCTATATACT ACGTATAGGA AATGTTTACA TTTTCGTATT
2341 GTTTTCGATT CACTCTATGA ATAGTTCTTA CTACAATTTT TTTGTCTAAA GAGTAATACT
2401 AGAGATAAAC ATAAAAAATG TAGAGGTCGA GTTTAGATGC AAGTTCAAGG AGCGAAAGGT
2461 GGATGGGTAG GTTATATAGG GATATAGCAC AGAGATATAT AGCAAAGAGA TACTTTTGAG
2521 CAATGTTTGT GGAAGCGGTA TTCGCAATAT TTTAGTAGCT CGTTACAGTC CGGTGCGTTT
2581 TTGGTTTTTT GAAAGTGCGT CTTCAGAGCG CTTTTGGTTT TCAAAAGCGC TCTGAAGTTC
2641 CTATACTTTC TAGCTAGAGA ATAGGAACTT CGGAATAGGA ACTTCAAAGC GTTTCCGAAA
2701 ACGAGCGCTT CCGAAAATGC AACGCGAGCT GCGCACATAC AGCTCACTGT TCACGTCGCA
2761 CCTATATCTG CGTGTTGCCT GTATATATAT ATACATGAGA AGAACGGCAT AGTGCGTGTT
2821 TATGCTTAAA TGCGTTATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA
2881 GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG
2941 CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC
3001 CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA
3061 ATGTCATGAT AATAATGGTT TCTTAGGGGG ATCTGTTTAG CTTGCCTCGT CCCCGCCGGG
3121 TCACCCGGCC AGCGACATGG AGGCCCAGAA TACCCTCCTT GACAGTCTTG ACGTGCGCAG
3181 CTCAGGGGCA TGATGTGACT GTCGCCCGTA CATTTAGCCC ATACATCCCC ATGTATAATC
3241 ATTTGCATCC ATACATTTTG ATGGCCGCAC GGCGCGAAGC AAAAATTACG GCTCCTCGCT
3301 GCAGACCTGC GAGCAGGGAA ACGCTCCCCT CACAGACGCG TTGAATTGTC CCCACGCCGC
3361 GCCCCTGTAG AGAAATATAA AAGGTTAGGA TTTGCCACTG AGGTTCTTCT TTCATATACT
3421 TCCTTTTAAA ATCTTGCTAG GATACAGTTC TCACATCACA TCCGAACATA AACAACCATG
3481 GGTAAGGAAA AGACTCACGT TTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA
3541 TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGATTG
3601 TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT
3661 GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC
3721 ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGC
3781 AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG
3841 CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC
3901 GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG
3961 AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT
4021 AAGCTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC
4081 CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA
4141 GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA
4201 CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT
4261 CATTTGATGC TCGATGAGTT TTTCTAATCA GTCCTCGGAG ATCCGTCCCC CTTTTCCTTT
4321 GTCGATATCA TGTAATTAGT TATGTCACGC TTACATTCAC GCCCTCCCCC CACATCCGCT
4381 CTAACCGAAA AGGAAGGAGT TAGACAACCT GAAGTCTAGG TCCCTATTTA TTTTTTTATA
4441 GTTATGTTAG TATTAAGAAC GTTATTTATA TTTCAAATTT TTCTTTTTTT TCTGTACAGA
4501 CGCGTGTACG CATGTAACAT TATACTGAAA ACCTTGCTTG AGAAGGTTTT GGGACGCTCG
4561 AAGGCTTTAA TTTGCAAGCT GGAGACCAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG
4621 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
4681 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
4741 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
4801 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG
4861 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
4921 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
4981 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
5041 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT
5101 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
5161 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
5221 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
5281 AACGAAAACT CACGTTAAGG GATTTTGGTC
//