Note: the two SalI sites are the only two sites on the plasmid.
pGilda complete sequence
LOCUS PGILDA.GB 6570 BP DS-DNA CIRCULAR SYN 05-OCT-2000
DEFINITION -
ACCESSION -
KEYWORDS -
SOURCE -
FEATURES Location/Qualifiers
promoter 2..671
/note="GAL1 promoter"
misc_feature 616..616
/note="transcription start site"
gene 692..1297
/note="LexA protein"
misc_feature 1298..1341
/note="MCS"
terminator 1363..1676
/note="ADH1 terminator"
rep_origin 1882..2320
/note="f1 bacteriophage origin"
gene complement(2598..3257)
/note="HIS3 gene"
rep_origin 3859..4377
/note="CEN/ARS"
gene 4460..5334
/note="Ampicillin"
rep_origin 5394..6274
/note="ColE1"
BASE COUNT 1794 A 1509 C 1473 G 1794 T 0 OTHER
ORIGIN -
1 CTTGAATTTT CAAAAATTCT TACTTTTTTT TTGGATGGAC GCAAAGAAGT TTAATAATCA
61 TATTACATGG CATTACCACC ATATACATAT CCATATACAT ATCCATATCT AATCTTACTT
121 ATATGTTGTG GAAATGTAAA GAGCCCCATT ATCTTAGCCT AAAAAAACCT TCTCTTTGGA
181 ACTTTCAGTA ATACGCTTAA CTGCTCATTG CTATATTGAA GTACGGATTA GAAGCCGCCG
241 AGCGGGTGAC AGCCCTCCGA AGGAAGACTC TCCTCCGTGC GTCCTCGTCT TCACCGGTCG
301 CGTTCCTGAA ACGCAGATGT GCCTCGCGCC GCACTGCTCC GAACAATAAA GATTCTACAA
361 TACTAGCTTT TATGGTTATG AAGAGGAAAA ATTGGCAGTA ACCTGGCCCC ACAAACCTTC
421 AAATGAACGA ATCAAATTAA CAACCATAGG ATGATAATGC GATTAGTTTT TTAGCCTTAT
481 TTCTGGGGTA ATTAATCAGC GAAGCGATGA TTTTTGATCT ATTAACAGAT ATATAAATGC
541 AAAAACTGCA TAACCACTTT AACTAATACT TTCAACATTT TCGGTTTGTA TTACTTCTTA
601 TTCAAATGTA ATAAAAGTAT CAACAAAAAA TTGTTAATAT ACCTCTATAC TTTAACGTCA
661 AGGAGAAAAA ACCCCGGATC AAGGGTGCGA TATGAAAGCG TTAACGGCCA GGCAACAAGA
721 GGTGTTTGAT CTCATCCGTG ATCACATCAG CCAGACAGGT ATGCCGCCGA CGCGTGCGGA
781 AATCGCGCAG CGTTTGGGGT TCCGTTCCCC AAACGCGGCT GAAGAACATC TGAAGGCGCT
841 GGCACGCAAA GGCGTTATTG AAATTGTTTC CGGCGCATCA CGCGGGATTC GTCTGTTGCA
901 GGAAGAGGAA GAAGGGTTGC CGCTGGTAGG TCGTGTGGCT GCCGGTGAAC CACTTCTGGC
961 GCAACAGCAT ATTGAAGGTC ATTATCAGGT CGATCCTTCC TTATTCAAGC CGAATGCTGA
1021 TTTCCTGCTG CGCGTCAGCG GGATGTCGAT GAAAGATATC GGCATTATGG ATGGTGACTT
1081 GCTGGCAGTG CATAAAACTC AGGATGTACG TAACGGTCAG GTCGTTGTCG CACGTATTGA
1141 TGACGAAGTT ACCGTTAAGC GCCTGAAAAA ACAGGGCAAT AAAGTCGAAC TGTTGCCAGA
1201 AAATAGCGAG TTTAAACCAA TTGTCGTAGA TCTTCGTCAG CAGAGCTTCA CCATTGAAGG
1261 GCTGGCGGTT GGGGTTATTC GCAACGGCGA CTGGCTGGAA TTCCCGGGGA TCCGTCGACC
1321 ATGGCGGCCG CTCGAGTCGA CCTGCAGCCA AGCTAATTCC GGGCGAATTT CTTATGATTT
1381 ATGATTTTTA TTATTAAATA AGTTATAAAA AAAATAAGTG TATACAAATT TTAAAGTGAC
1441 TCTTAGGTTT TAAAACGAAA ATTCTTGTTC TTGAGTAACT CTTTCCTGTA GGTCAGGTTG
1501 CTTTCTCAGG TATAGCATGA GGTCGCTCTT ATTGACCACA CCTCTACCGG CATGCCGAGC
1561 AAATGCCTGC AAATCGCTCC CCATTTCACC CAATTGTAGA TATGCTAACT CCAGCAATGA
1621 GTTGATGAAT CTCGGTGTGT ATTTTATGTC CTCAGAGGAC AACACCTGTT GTAATCCGTC
1681 CGAGCTCCAA TTCGCCCTAT AGTGAGTCGT ATTACAATTC ACTGGCCGTC GTTTTACAAC
1741 GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT
1801 TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA
1861 GCCTGAATGG CGAATGGCGC GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG
1921 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT
1981 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC
2041 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG
2101 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG
2161 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT
2221 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG
2281 AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCCT
2341 GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT GATCCGTCGA
2401 GTTCAAGAGA AAAAAAAAGA AAAAGCAAAA AGAAAAAAGG AAAGCGCGCC TCGTTCAGAA
2461 TGACACGTAT AGAATGATGC ATTACCTTGT CATCTTCAGT ATCATACTGT TCGTATACAT
2521 ACTTACTGAC ATTCATAGGT ATACATATAT ACACATGTAT ATATATCGTA TGCTGCAGCT
2581 TTAAATAATC GGTGTCACTA CATAAGAACA CCTTTGGTGG AGGGAACATC GTTGGTACCA
2641 TTGGGCGAGG TGGCTTCTCT TATGGCAACC GCAAGAGCCT TGAACGCACT CTCACTACGG
2701 TGATGATCAT TCTTGCCTCG CAGACAATCA ACGTGGAGGG TAATTCTGCT AGCCTCTGCA
2761 AAGCTTTCAA GAAAATGCGG GATCATCTCG CAAGAGAGAT CTCCTACTTT CTCCCTTTGC
2821 AAACCAAGTT CGACAACTGC GTACGGCCTG TTCGAAAGAT CTACCACCGC TCTGGAAAGT
2881 GCCTCATCCA AAGGCGCAAA TCCTGATCCA AACCTTTTTA CTCCACGCGC CAGTAGGGCC
2941 TCTTTAAAAG CTTGACCGAG AGCAATCCCG CAGTCTTCAG TGGTGTGATG GTCGTCTATG
3001 TGTAAGTCAC CAATGCACTC AACGATTAGC GACCAGCCGG AATGCTTGGC CAGAGCATGT
3061 ATCATATGGT CCAGAAACCC TATACCTGTG TGGACGTTAA TCACTTGCGA TTGTGTGGCC
3121 TGTTCTGCTA CTGCTTCTGC CTCTTTTTCT GGGAAGATCG AGTGCTCTAT CGCTAGGGGA
3181 CCACCCTTTA AAGAGATCGC AATCTGAATC TTGGTTTCAT TTGTAATACG CTTTACTAGG
3241 GCTTTCTGCT CTGTCATCTT TGCCTTCGTT TATCTTGCCT GCTCATTTTT TAGTATATTC
3301 TTCGAAGAAA TCACATTACT TTATATAATG TATAATTCAT TATGTGATAA TGCCAATCGC
3361 TAAGAAAAAA AAAGAGTCAT CCGCTAGGTG GAAAAAAAAA AATGAAAATC ATTACCGAGG
3421 CATAAAAAAA TATAGAGTGT ACTAGAGGAG GCCAAGAGTA ATAGAAAAAG AAAATTGCGG
3481 GAAAGGACTG TGTTATGACT TCCCTGACTA ATGCCGTGTT CAAACGATAC CTGGCAGTGA
3541 CTCCTAGCGC TCACCAAGCT CTTAAAACGG AATTATGGTG CACTCTCAGT ACAATCTGCT
3601 CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC
3661 GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA
3721 TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC
3781 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGGACGGA TCGCTTGCCT
3841 GTAACTTACA CGCGCCTCGT ATCTTTTAAT GATGGAATAA TTTGGGAATT TACTCTGTGT
3901 TTATTTATTT TTATGTTTTG TATTTGGATT TTAGAAAGTA AATAAAGAAG GTAGAAGAGT
3961 TACGGAATGA AGAAAAAAAA ATAAACAAAG GTTTAAAAAA TTTCAACAAA AAGCGTACTT
4021 TACATATATA TTTATTAGAC AAGAAAAGCA GATTAAATAG ATATACATTC GATTAACGAT
4081 AAGTAAAATG TAAAATCACA GGATTTTCGT GTGTGGTCTT CTACACAGAC AAGATGAAAC
4141 AATTCGGCAT TAATACCTGA GAGCAGGAAG AGCAAGATAA AAGGTAGTAT TTGTTGGCGA
4201 TCCCCCTAGA GTCTTTTACA TCTTCGGAAA ACAAAAACTA TTTTTTCTTT AATTTCTTTT
4261 TTTACTTTCT ATTTTTAATT TATATATTTA TATTAAAAAA TTTAAATTAT AATTATTTTT
4321 ATAGCACGTG ATGAAAAGGA CCCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT
4381 ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA
4441 TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC
4501 CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG
4561 AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC
4621 AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT
4681 TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC
4741 GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG
4801 CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT
4861 AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT
4921 TTTCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA
4981 GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC
5041 AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG
5101 GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT
5161 GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA
5221 GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GCAGTCAGGC AACTATGGAT
5281 GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA
5341 GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG
5401 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG
5461 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT
5521 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG
5581 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA
5641 CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA
5701 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG
5761 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC
5821 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA
5881 TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG
5941 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGGAAC
6001 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG
6061 TGATGCTCGT CAGGGGGGCC GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG
6121 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT
6181 GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC
6241 GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC
6301 CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG
6361 GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA
6421 CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA
6481 GGAAACAGCT ATGACCATGA TTACCCCAAG CTCGAAATTA ACCCTCACTA AAGGGAACAA
6541 AAGCTGGTAC CGGGCCCCCC CTCGAAATTC
//