pHybLex/Zeo complete sequence
LOCUS PHYBLEX/ZE 4765 BP DS-DNA SYN 23-MAY-2000
DEFINITION -
ACCESSION -
KEYWORDS -
SOURCE -
FEATURES Location/Qualifiers
promoter 1..399
/note="ADh1 promoter"
misc_feature 420..1029
/note="LexA ORF"
misc_feature 1030..1093
/note="MCS"
primer_bind 986..1010
/note="pHybLex/Zeo forward priming site"
primer_bind 1161..1185
/note="pHybLex/Zeo reverse priming site"
terminator 1144..1301
/note="ADH1 transcription termination region"
rep_origin 1602..2311
/note="2µ origin"
promoter 2858..3266
/note="TEF1 promoter"
promoter 3270..3337
/note="EM-7 promoter"
gene 3338..3712
/note="Zeocin resistance gene"
terminator 3713..4030
/note="CYC1 transcription termination regions"
rep_origin 4041..4714
/note="pMB1 (pUC-derived) origin"
BASE COUNT 1214 A 1110 C 1085 G 1356 T 0 OTHER
ORIGIN -
1 CAACTTCTTT TCTTTTTTTT TCTTTTCTCT CTCCCCCGTT GTTGTCTCAC CATATCCGCA
61 ATGACAAAAA AAATGATGGA AGACACTAAA GGAAAAAATT AACGACAAAG ACAGCACCAA
121 CAGATGTCGT TGTTCCAGAG CTGATGAGGG GTATCTTCGA ACACACGAAA CTTTTTCCTT
181 CCTTCATTCA CGCACACTAC TCTCTAATGA GCAACGGTAT ACGGCCTTCC TTCCAGTTAC
241 TTGAATTTGA AATAAAAAAA GTTTGCCGCT TTGCTATCAA GTATAAATAG ACCTGCAATT
301 ATTAATCTTT TGTTTCCTCG TCATTGTTCT CGTTCCCTTT CTTCCTTGTT TCTTTTTCTG
361 CACAATATTT CAAGCTATAC CAAGCATACA ATCAACTCCA AGCTTTAATT CCGGGCGGAA
421 TGAAAGCGTT AACGGCCAGG CAACAAGAGG TGTTTGATCT CATCCGTGAT CACATCAGCC
481 AGACAGGTAT GCCGCCGACG CGTGCGGAAA TCGCGCAGCG TTTGGGGTTC CGTTCCCCAA
541 ACGCGGCTGA AGAACATCTG AAGGCGCTGG CACGCAAAGG CGTTATTGAA ATTGTTTCCG
601 GCGCATCACG CGGGATTCGT CTGTTGCAGG AAGAGGAAGA AGGGTTGCCG CTGGTAGGTC
661 GTGTGGCTGC CGGTGAACCA CTTCTGGCGC AACAGCATAT TGAAGGTCAT TATCAGGTCG
721 ATCCTTCCTT ATTCAAGCCG AATGCTGATT TCCTGCTGCG CGTCAGCGGG ATGTCGATGA
781 AAGATATCGG CATTATGGAT GGTGACTTGC TGGCAGTGCA TAAAACTCAG GATGTACGTA
841 ACGGTCAGGT CGTTGTCGCA CGTATTGATG ACGAAGTTAC CGTTAAGCGC CTGAAAAAAC
901 AGGGCAATAA AGTCGAACTG TTGCCAGAAA ATAGCGAGTT TAAACCAATT GTCGTAGATC
961 TTCGTCAGCA GAGCTTCACC ATTGAAGGGC TGGCGGTTGG GGTTATTCGC AACGGCGACT
1021 GGCTGGAATT CAAGCTTGAG CTCAGATCTC AGCTGGGCCC GGTACCGCGG CCGCTCGAGT
1081 CGACCTGCAG CCAAGCTAAT TCCGGGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA
1141 ATAAGTTATA AAAAAAATAA GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG
1201 AAAATTCTTG TTCTTGAGTA ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGCA
1261 TGAGGTCGCT CTTATTGACC ACACCTCTAC CGGCATGCCG AGCAAATGCC TGCAAATCGC
1321 TCCCCATTTC ACCCAATTGT AGATATGCTA ACTCCAGCAA TGAGTTGATG AATCTCGGTG
1381 TGTATTTTAT GTCCTCAGAG GACAATACCT GTTGTAATCC GTCCCAAGCT AACGAAGCAT
1441 CTGTGCTTCA TTTTGTAGAA CAAAAATGCA ACGCGAGAGC GCTAATTTTT CAAACAAAGA
1501 ATCTGAGCTG CATTTTTACA GAACAGAAAT GCAACGCGAA AGCGCTATTT TACCAACGAA
1561 GAATCTGTGC TTCATTTTTG TAAAACAAAA ATGCAACGCG AGAGCGCTAA TTTTTCAAAC
1621 AAAGAATCTG AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAGAGCGC TATTTTACCA
1681 ACAAAGAATC TATACTTCTT TTTTGTTCTA CAAAAATGCA TCCCGAGAGC GCTATTTTTC
1741 TAACAAAGCA TCTTAGATTA CTTTTTTTCT CCTTTGTGCG CTCTATAATG CAGTCTCTTG
1801 ATAACTTTTT GCACTGTAGG TCCGTTAAGG TTAGAAGAAG GCTACTTTGG TGTCTATTTT
1861 CTCTTCCATA AAAAAAGCCT GACTCCACTT CCCGCGTTTA CTGATTACTA GCGAAGCTGC
1921 GGGTGCATTT TTTCAAGATA AAGGCATCCC CGATTATATT CTATACCGAT GTGGATTGCG
1981 CATACTTTGT GAACAGAAAG TGATAGCGTT GATGATTCTT CATTGGTCAG AAAATTATGA
2041 ACGGTTTCTT CTATTTTGTC TCTATATACT ACGTATAGGA AATGTTTACA TTTTCGTATT
2101 GTTTTCGATT CACTCTATGA ATAGTTCTTA CTACAATTTT TTTGTCTAAA GAGTAATACT
2161 AGAGATAAAC ATAAAAAATG TAGAGGTCGA GTTTAGATGC AAGTTCAAGG AGCGAAAGGT
2221 GGATGGGTAG GTTATATAGG GATATAGCAC AGAGATATAT AGCAAAGAGA TACTTTTGAG
2281 CAATGTTTGT GGAAGCGGTA TTCGCAATAT TTTAGTAGCT CGTTACAGTC CGGTGCGTTT
2341 TTGGTTTTTT GAAAGTGCGT CTTCAGAGCG CTTTTGGTTT TCAAAAGCGC TCTGAAGTTC
2401 CTATACTTTC TAGCTAGAGA ATAGGAACTT CGGAATAGGA ACTTCAAAGC GTTTCCGAAA
2461 ACGAGCGCTT CCGAAAATGC AACGCGAGCT GCGCACATAC AGCTCACTGT TCACGTCGCA
2521 CCTATATCTG CGTGTTGCCT GTATATATAT ATACATGAGA AGAACGGCAT AGTGCGTGTT
2581 TATGCTTAAA TGCGTTATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA
2641 GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG
2701 CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC
2761 CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA
2821 ATGTCATGAT AATAATGGTT TCTTAGGGGG ATCCCCCACA CACCATAGCT TCAAAATGTT
2881 TCTACTCCTT TTTTACTCTT CCAGATTTTC TCGGACTCCG CGCATCGCCG TACCACTTCA
2941 AAACACCCAA GCACAGCATA CTAAATTTTC CCTCTTTCTT CCTCTAGGGT GTCGTTAATT
3001 ACCCGTACTA AAGGTTTGGA AAAGAAAAAA GAGACCGCCT CGTTTCTTTT TCTTCGTCGA
3061 AAAAGGCAAT AAAAATTTTT ATCACGTTTC TTTTTCTTGA AATTTTTTTT TTTAGTTTTT
3121 TTCTCTTTCA GTGACCTCCA TTGATATTTA AGTTAATAAA CGGTCTTCAA TTTCTCAAGT
3181 TTCAGTTTCA TTTTTCTTGT TCTATTACAA CTTTTTTTAC TTCTTGTTCA TTAGAAAGAA
3241 AGCATAGCAA TCTAATCTAA GGGGCGGTGT TGACAATTAA TCATCGGCAT AGTATATCGG
3301 CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC AAGTTGACCA GTGCCGTTCC
3361 GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC TGGACCGACC GGCTCGGGTT
3421 CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC CGGGACGACG TGACCCTGTT
3481 CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC CTGGCCTGGG TGTGGGTGCG
3541 CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG TCCACGAACT TCCGGGACGC
3601 CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG
3661 CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG CAGGACTGAC ACGTCCGACG
3721 GCGGCCCACG GGTCCCAGGC CTCGGAGATC CGTCCCCCTT TTCCTTTGTC GATATCATGT
3781 AATTAGTTAT GTCACGCTTA CATTCACGCC CTCCCCCCAC ATCCGCTCTA ACCGAAAAGG
3841 AAGGAGTTAG ACAACCTGAA GTCTAGGTCC CTATTTATTT TTTTATAGTT ATGTTAGTAT
3901 TAAGAACGTT ATTTATATTT CAAATTTTTC TTTTTTTTCT GTACAGACGC GTGTACGCAT
3961 GTAACATTAT ACTGAAAACC TTGCTTGAGA AGGTTTTGGG ACGCTCGAAG GCTTTAATTT
4021 GCAAGCTGGA GACCAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG
4081 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC
4141 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
4201 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT
4261 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG
4321 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
4381 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC
4441 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT
4501 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC
4561 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA
4621 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
4681 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC
4741 GTTAAGGGAT TTTGGTCATG AGATC
//