pYesTrp2 complete sequence
LOCUS PYESTRP2A. 5823 BP DS-DNA CIRCULAR SYN 23-FEB-2001
DEFINITION -
ACCESSION -
KEYWORDS -
SOURCE -
FEATURES Location/Qualifiers
gene complement(5626..5823)
/note="ampicillin"
gene complement(1..663)
/note="ampicillin"
promoter complement(664..762)
/note="bla promoter"
promoter 871..972
/note="TRP1 promoter"
gene 973..1647
/note="TRP1 gene"
rep_origin 2051..2885
/note="2µ ori"
rep_origin complement(2954..3326)
/note="fi ori"
promoter 3381..3878
/note="GAL1 promoter"
promoter 3902..3921
/note="T7 promoter/priming site"
misc_feature 3937..3939
/note="Initiation ATG"
misc_binding 3940..3981
/note="V5 Epitope"
misc_feature 3997..4023
/note="NLS"
misc_feature 4027..4264
/note="B42 Activation Domain"
misc_feature 4270..4363
/note="MCS"
terminator 4378..4626
/note="CYC1 term."
rep_origin complement(4808..5481)
/note="pMB1 origin"
BASE COUNT 1642 A 1260 C 1335 G 1586 T 0 OTHER
ORIGIN -
1 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
61 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC
121 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
181 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
241 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
301 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
361 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAC
421 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
481 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
541 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
601 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
661 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG
721 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG
781 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG
841 GCGTATCACG AGGCCCTTTC GTCTTCAAGA AATTCGGTCG AAAAAAGAAA AGGAGAGGGC
901 CAAGAGGGAG GGCATTGGTG ACTATTGAGC ACGTGAGTAT ACGTGATTAA GCACACAAAG
961 GCAGCTTGGA GTATGTCTGT TATTAATTTC ACAGGTAGTT CTGGTCCATT GGTGAAAGTT
1021 TGCGGCTTGC AGAGCACAGA GGCCGCAGAA TGTGCTCTAG ATTCCGATGC TGACTTGCTG
1081 GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC CGGTTATTGC AAGGAAAATT
1141 TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC CGAAATACTT GGTTGGCGTG
1201 TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA ATGATTACGG CATTGATATC
1261 GTCCAACTGC ACGGAGATGA GTCGTGGCAA GAATACCAAG AGTTCCTCGG TTTGCCAGTT
1321 ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC TCAGTGCAGC TTCACAGAAA
1381 CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG GGACAGGTGA ACTTTTGGAT
1441 TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC CCGAGAGCTT ACATTTTATG
1501 TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC TTAGATTAAA TGGCGTTATT
1561 GGTGTTGATG TAAGCGGAGG TGTGGAGACA AATGGTGTAA AAGACTCTAA CAAAATAGCA
1621 AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT AGTATTTATT TAAGTATTGT
1681 TTGTGCACTT GCCTGCAGCT TCTCAATGAT ATTCGAATAC GCTTTGAGGA GATACAGCCT
1741 AATATCCGAC AAACTGTTTT ACAGATTTAC GATCGTACTT GTTACCCATC ATTGAATTTT
1801 GAACATCCGA ACCTGGGAGT TTTCCCTGAA ACAGATAGTA TATTTGAACC TGTATAATAA
1861 TATATAGTCT AGCGCTTTAC GGAAGACAAT GTATGTATTT CGGTTCCTGG AGAAACTATT
1921 GCATCTATTG CATAGGTAAT CTTGCACGTC GCATCCCCGG TTCATTTTCT GCGTTTCCAT
1981 CTTGCACTTC AATAGCATAT CTTTGTTAAC GAAGCATCTG TGCTTCATTT TGTAGAACAA
2041 AAATGCAACG CGAGAGCGCT AATTTTTCAA ACAAAGAATC TGAGCTGCAT TTTTACAGAA
2101 CAGAAATGCA ACGCGAAAGC GCTATTTTAC CAACGAAGAA TCTGTGCTTC ATTTTTGTAA
2161 AACAAAAATG CAACGCGAGA GCGCTAATTT TTCAAACAAA GAATCTGAGC TGCATTTTTA
2221 CAGAACAGAA ATGCAACGCG AGAGCGCTAT TTTACCAACA AAGAATCTAT ACTTCTTTTT
2281 TGTTCTACAA AAATGCATCC CGAGAGCGCT ATTTTTCTAA CAAAGCATCT TAGATTACTT
2341 TTTTTCTCCT TTGTGCGCTC TATAATGCAG TCTCTTGATA ACTTTTTGCA CTGTAGGTCC
2401 GTTAAGGTTA GAAGAAGGCT ACTTTGGTGT CTATTTTCTC TTCCATAAAA AAAGCCTGAC
2461 TCCACTTCCC GCGTTTACTG ATTACTAGCG AAGCTGCGGG TGCATTTTTT CAAGATAAAG
2521 GCATCCCCGA TTATATTCTA TACCGATGTG GATTGCGCAT ACTTTGTGAA CAGAAAGTGA
2581 TAGCGTTGAT GATTCTTCAT TGGTCAGAAA ATTATGAACG GTTTCTTCTA TTTTGTCTCT
2641 ATATACTACG TATAGGAAAT GTTTACATTT TCGTATTGTT TTCGATTCAC TCTATGAATA
2701 GTTCTTACTA CAATTTTTTT GTCTAAAGAG TAATACTAGA GATAAACATA AAAAATGTAG
2761 AGGTCGAGTT TAGATGCAAG TTCAAGGAGC GAAAGGTGGA TGGGTAGGTT ATATAGGGAT
2821 ATAGCACAGA GATATATAGC AAAGAGATAC TTTTGAGCAA TGTTTGTGGA AGCGGTATTC
2881 GCAATGGGAA GCTCCACCCC GGTTGATAAT CAGAAAAGCC CCAAAAACAG GAAGATTGTA
2941 TAAGCAAATA TTTAAATTGT AAACGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT
3001 TAAATCAGCT CATTTTTTAA CGAATAGCCC GAAATCGGCA AAATCCCTTA TAAATCAAAA
3061 GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTCCA ACAAGAGTCC ACTATTAAAG
3121 AACGTGGACT CCAACGTCAA AGGGCGAAAA AGGGTCTATC AGGGCGATGG CCCACTACGT
3181 GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCAGT AAATCGGAAG
3241 GGTAAACGGA TGCCCCCATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG
3301 GAAGGGAAGA AAGCGAAAGG AGCGGGGGCT AGGGCGGTGG GAAGTGTAGG GGTCACGCTG
3361 GGCGTAACCA CCACACCCGC CGCGCTTAAT GGGGCGCTAC AGGGCGCGTG GGGATGATCC
3421 ACTAGTACGG ATTAGAAGCC GCCGAGCGGG TGACAGCCCT CCGAAGGAAG ACTCTCCTCC
3481 GTGCGTCCTC GTCCTCACCG GTCGCGTTCC TGAAACGCAG ATGTGCCTCG CGCCGCACTG
3541 CTCCGAACAA TAAAGATTCT ACAATACTAG CTTTTATGGT TATGAAGAGG AAAAATTGGC
3601 AGTAACCTGG CCCCACAAAC CTTCAAATGA ACGAATCAAA TTAACAACCA TAGGATGATA
3661 ATGCGATTAG TTTTTTAGCC TTATTTCTGG GGTAATTAAT CAGCGAAGCG ATGATTTTTG
3721 ATCTATTAAC AGATATATAA ATGCAAAAAC TGCATTAACC ACTTTAACTA ATACTTTCAA
3781 CATTTTCGGT TTGTATTACT TCTTATTCAA ATGTAATAAA AGTATCAACA AAAAATTGTT
3841 AATATACCTC TATACTTTAA CGTCAAGGAG AAAAAACCCC GGATCGGACT ACTAGCAGCT
3901 GTAATACGAC TCACTATAGG GAATATTAAG CTCACCATGG GTAAGCCTAT CCCTAACCCT
3961 CTCCTCGGTC TCGATTCTAC ACAAGCTATG GGTGCTCCTC CAAAAAAGAA GAGAAAGGTA
4021 GCTGGTATCA ATAAAGATAT CGAGGAGTGC AATGCCATCA TTGAGCAGTT TATCGACTAC
4081 CTGCGCACCG GACAGGAGAT GCCGATGGAA ATGGCGGATC AGGCGATTAA CGTGGTGCCG
4141 GGCATGACGC CGAAAACCAT TCTTCACGCC GGGCCGCCGA TCCAGCCTGA CTGGCTGAAA
4201 TCGAATGGTT TTCATGAAAT TGAAGCGGAT GTTAACGATA CCAGCCTCTT GCTGAGTGGA
4261 GATGCCTCCA AGCTTGGTAC CGAGCTCGGA TCCACTAGTA ACGGCCGCCA GTGTGCTGGA
4321 ATTCTGCAGA TATCCATCAC ACTGGCGGCC GCTCGAGGCA TGCATCTAGA GGGCCGCATC
4381 ATGTAATTAG TTATGTCACG CTTACATTCA CGCCCTCCCC CCACATCCGC TCTAACCGAA
4441 AAGGAAGGAG TTAGACAACC TGAAGTCTAG GTCCCTATTT ATTTTTTTAT AGTTATGTTA
4501 GTATTAAGAA CGTTATTTAT ATTTCAAATT TTTCTTTTTT TTCTGTACAG ACGCGTGTAC
4561 GCATGTAACA TTATACTGAA AACCTTGCTT GAGAAGGTTT TGGGACGCTC GAAGGCTTTA
4621 ATTTGCGGCC CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG
4681 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC
4741 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG
4801 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GCCCAGGAAC CGTAAAAAGG CCGCGTTGCT
4861 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
4921 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
4981 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
5041 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
5101 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
5161 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
5221 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
5281 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
5341 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
5401 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
5461 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
5521 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
5581 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
5641 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
5701 CGTCGTGTAG ATAACTACGA TACGGGAGCG CTTACCATCT GGCCCCAGTG CTGCAATGAT
5761 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
5821 GGC
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