Faculty Summaries
Luis J Sigal, DVM, PhD
Luis J. Sigal, DVM, PhD
  • Adjunct Assistant Professor of Microbiology, University of Pennsylvania School of Medicine
  • Adjunct Associate Professor, Microbiology & Immunology, Thomas Jefferson University
Office Phone: 215-728-7061
Lab Phone: 215-728-7065,7067,7020
Fax: 215-728-2412
Office: W260


  • Rubio, D., Xu, R.-H., Remakus, S., Krouse, T.E., Truckenmiller, M.E., Thapa, R., Balachandran, S., Alcami, A., Norbury, C.C., Sigal, L.J. Cross-talk between the Type I interferon and Nuclear Factor Kappa B pathways rescues resistance to a viral disease. Cell Host Microbe 2013;13:701-10. PubMed
  • Remakus S, Rubio D, Lev A, Ma X, Fang M, Xu R-H, Sigal, LJ. Memory CD8 T cells can outsource IFN-γ production but not cytolytic killing for anti-viral protection. Cell Host Microbe 2013;13:546-57. PubMed
  • Ma X, Xu R-H, Roscoe F, Whitbeck JC, Eisenberg RJ, Cohen GH, Sigal LJ. The mature virion of a pathogenic orthopoxvirus is important for late spread in vivo serving as a target for delayed therapy. J Virol. 2013;87:7046-53. PubMed
  • Roscoe F, Xu R-H, Sigal LJ. Characterization of Ectromelia virus deficient in EVM036, the homolog of Vaccinia virus F13L, and its application for the rapid generation of recombinant viruses. J Virol. 2012;86:13501-7. PubMed
  • Remakus S, Rubio D, Ma X, Sette A, Sigal LJ. Memory CD8 T cells specific for a single immmunodominant or subdominant determinant induced by peptide-dendritic cell immunization protect from an acute lethal viral disease. J Virol. 2012;86:9748-59. PubMed
  • Fang M, Siciliano NA, Hersperger AR, Roscoe F, Hu A, Ma X, Shamsedeen AR, Eisenlohr LC, Sigal LJ. Perforin-dependent CD4+ T-cell cytotoxicity contributes to control a murine poxvirus infection. Proc Natl Acad Sci USA. 2012;109:9983-8. PubMed
  • Xu R-H, Rubio D, Roscoe F, Krouse TE, Truckenmiller ME, Norbury CC, Hudson PN, Damon IK, Alcami A, Sigal LJ. Antibody inhibition of a viral type 1 interferon decoy receptor cures a viral disease by restoring interferon signaling in the liver. PLoS Pathogens. 2012;8:e1002475. PubMed
  • Remakus S, Sigal LJ. Interferon gamma and perforin control the strength, but not the hierarchy, of immunodominance of an anti-viral CD8 T cell response. J Virol. 2011;85:12578-84. PubMed
  • Fang M, Orr MT, Spee P, Egebjerg T, Lanier LL, Sigal LJ. CD94 is essential for NK cell-mediated resistance to a lethal viral disease. Immunity 2011;34:579-89. PubMed
  • Orr MT, Wu J, Fang M, Sigal LJ, Spee P, Egebjerg T, Dissen E, Fossum S, Phillips JH, Lanier LL. Development and function of CD94-deficient natural killer cells. PLoS ONE 2010;5:e15184. PubMed
  • Fang M, Roscoe F, Sigal LJ. Age-dependent susceptibility to a viral disease due to decreased natural killer cell numbers and trafficking. J Exp Med 2010;207:2369-81. PubMed
  • Fang M, Sigal LJ. Studying NK cell responses to ectromelia virus infections in mice. In: Natural Killer Cell Protocols, Methods in Molecular Biology Series (Campbell KS, ed), 2nd Edition, Chapter 28, vol. 612, pp. 411-428. Humana Press, 2010.
  • Xu R-H, Remakus S, Ma X, Roscoe F et al. Direct presentation is sufficient for an efficient anti-viral CD8+ T cell response. PLoS Pathog 2010;6:e1000768. PubMed
  • Ma X, Serna A, Xu RH et al. The amino acids sequences flanking an antigentic determinant can strongly affect MHC class I cross-presentation without altering direct-presentation. J Immunol 2009;182(8):4601-07. PubMed
  • Xu RH, Cohen M, Tang Y et al. The orthopoxvirus type I IFN binding protein is essential for virulence and an effective target for vaccination. J Exp Med. 2008;205(4):981-92. PubMed
  • MacFarlane AW, Yamazaki T, Fang M et al. Enhanced NK-cell development and function in BCAP-deficient mice. Blood. 2008;112(1):131-40. PubMed
  • Fang M, Lanier LL, Sigal LJ. A role for NKG2D in NK cell-mediated resistance to poxvirus disease. PLoS Pathog. 2008;4(2):e30. PubMed
  • Xu R-H, Fang M, Klein-Szanto A et al. Memory CD8+ T cells are gatekeepers of the lymph node draining the site of viral infection. Proc Natl Acad Sci U S A. 2007;104(26):10992-7. PubMed
  • Fang M, Sigal LJ. Direct CD28 costimulation is required for CD8+ T cell-mediated resistance to an acute viral disease in a natural host. J Immunol. 2006;177(11):8027-36. PubMed
  • Fang M, Cheng H, Dai Z et al. Immunization with a single extracellular enveloped virus protein produced in bacteria provides partial protection from a lethal orthopoxvirus infection in a natural host. Virology. 2006;345(1):231-43. PubMed
  • Fang M, Sigal LJ. Antibodies and CD8+ T cells are complementary and essential for natural resistance to a highly lethal cytopathic virus. J Immunol. 2005;175(10):6829-36. PubMed
  • Shen L, Sigal LJ, Boes M et al. Important role of cathepsin S in generating peptides for TAP-independent MHC class I crosspresentation in vivo. Immunity. 2004;21(2):155-65. PubMed
  • Ramirez MC, Sigal LJ. The multiple routes of MHC-I cross-presentation. Trends Microbiol. 2004;12(5):204-7. PubMed
  • Serna A, Ramirez MC, Soukhanova A et al. Cutting Edge: Efficient MHC class I cross-presentation during early vaccinia infection requires the transfer of proteasomal intermediates between antigen donor and presenting cells. J Immunol. 2003;171(11):5668-72. PubMed
  • Norbury CC, Sigal LJ. Cross priming or direct priming: is that really the question? Curr Opin Immunol. 2003;15(1):82-8.
  • Ramirez MC, Sigal LJ. Macrophages and dendritic cells use the cytosolic pathway to rapidly cross-present antigen from live, vaccinia-infected cells. J Immunol. 2002;169(12):6733-42. PubMed
  • Crotty S, Hix L, Sigal LJ et al. Poliovirus pathogenesis in a new poliovirus receptor transgenic mouse model: age-dependent paralysis and a mucosal route of infection. J Gen Virol. 2002;83(Pt 7):1707-20. PubMed
  • Chang J, Sigal LJ, Lerro A et al. Replication of the human hepatitis delta virus genome is initiated in mouse hepatocytes following intravenous injection of naked DNA or RNA sequences. J Virol. 2001;75(7):3469-73. PubMed
  • Sigal LJ, Rock KL. Bone marrow-derived antigen-presenting cells are required for the generation of cytotoxic T lymphocyte responses to viruses and use transporter associated with antigen presentation (TAP)-dependent and -independent pathways of antigen presentation. J Exp Med. 2000;192(8):1143-50. PubMed
  • Sigal LJ, Crotty S, Andino R et al. Cytotoxic T-cell immunity to virus-infected non-haematopoietic cells requires presentation of exogenous antigen. Nature. 1999;398(6722):77-80. PubMed
  • Sigal LJ, Reiser H, Rock KL. The role of B7-1 and B7-2 costimulation for the generation of CTL responses in vivo. J Immunol. 1998;161(6):2740-5. PubMed
  • Mandl S, Sigal LJ, Rock KL et al. Poliovirus vaccine vectors elicit antigen-specific cytotoxic T cells and protect mice against lethal challenge with malignant melanoma cells expressing a model antigen. Proc Natl Acad Sci U S A. 1998;95(14):8216-21. PubMed
  • Sigal LJ, Wylie DE. Role of non-anchor residues of Db-restricted peptides in class I binding and TCR triggering. Mol Immunol. 1996;33(17-18):1323-33. PubMed
  • Sigal LJ, Goebel P, Wylie DE. Db-binding peptides from influenza virus: effect of non-anchor residues on stability and immunodominance. Molecular Immunology. 1995;32(9):623-32. PubMed
  • Sigal LJ, Wylie DE. A semiquantitative rosetting assay for detection of cell-surface class I major histocompatibility complex molecules. Biotechniques. 1994;17(4):776-80. PubMed
  • Sigal LJ, Berens S, Wylie D. A lactate dehydrogenase (LDH)-based immunoassay for detection of cell surface antigens and its application to the study of MHC class I-binding peptides. Journal of Immunological Methods. 1994;177(1-2):261-8. PubMed
  • Sigal LJ, Gomez G, Braun M. Changes in mononuclear peripheral blood cells in cattle with foot-and-mouth disease. Veterinary Immunology & Immunopathology. 1992;30(4):431-8. PubMed
  • Braun M, Sigal L, Mundo S et al. Immune response against foot-and-mouth disease virus in cattle: effect of vaccination. Medicina. 1989;49(3):216-20. PubMed