Faculty Summaries
Dr. Skalka
Ann Skalka, PhD
Senior Advisor to the President
  • Professor
  • W.W. Smith Chair in Cancer Research
AM_Skalka@fccc.edu
Office Phone: 215-728-2490
Fax: 215 728 2778
Office: R419
Research Interests

 

  • Molecular aspects of viral replication, focus on human (HIV) and avian (ASLV) retroviruses.
  • Structure and function of retroviral integrase.
  • Epigenetic control of retroviral gene expression.
Description of research projects
Selected Publications
    1. Skalka, A.M. Retroviral DNA transposition: Themes and variations. In: Mobile DNA III (Sandmeyer, S., Craig, N., eds.). ASM Press, in press, 2015. Available in Microbiology Link
    2. Benleulmi, M.S., Matysiak, J., Henriquez, D.R., Vaillant, C., Lesbats, P., Calmels, C., Naughtin, M., Leon, O., Skalka, A.M., Ruff, M., Lavigne, M., Andreola, M.L., Parissi, V. Intasome architecture and chromatin density modulate retroviral integration into nucleosome. Retrovirology 12:13-29, 2015. PMCID: PMC4358916 PubMed
    3. Haugh, K.A., Shalginskikh, N., Nogusa, S., Skalka, A.M., Katz, R.A., Balachandran, S. The interferon-inducible antiviral protein Daxx is not essential for interferon-mediated protection against avian sarcoma virus. Virol. J. 11:100, 2014. PMCID: PMC4049388 PubMed
    4. Poleshko, A., Kossenkov, A.V., Shalginskikh, N., Pecherskaya, A., Einarson, M.B., Skalka, A.M., Katz, R.A. Human factors and pathways essential for mediating epigenetic gene silencing. Epigenetics 9:1280-1289, 2014. PMCID: PMC4169020 PubMed
    5. Bojja, R.S., Andrake, M.D., Merkel, G., Weigand, S., Dunbrack, R.L., Jr., Skalka, A.M. Architecture and assembly of HIV integrase multimers in the absence of DNA substrates. J. Biol. Chem. 288:7373-7386, 2013. PMCID: PMC3591645 PubMed
    6. Shalginskikh, N., Poleshko, A., Skalka, A.M., Katz, R.A. Retroviral DNA methylation and epigenetic repression are mediated by the antiviral host protein Daxx. Selected by the Editors as an Article of "Significant Interest". J. Virol. 87:2137-2150, 2013. PMCID: PMC3571491 PubMed
    7. Peletskaya, E., Andrake, M., Gustchina, A., Merkel, G., Alexandratos, J., Zhou, D., Bojja, R.S., Satoh, T., Potapov, M., Kogon, A., Potapov, V., Wlodawer, A., Skalka, A.M. Localization of ASV integrase-DNA contacts by site-directed crosslinking and their structural analysis. PLoS One 6:e27751, 2011. PMCID: PMC3228729 PubMed
    8. Katz, R.A., Merkel, G., Andrake, M.D., Roder, H., Skalka, A.M. Retroviral integrases promote fraying of viral DNA ends. J. Biol. Chem. 286:25710-25718, 2011. PMCID: PMC3138259 PubMed
    9. Bojja, R.S., Andrake, M.D., Weigand, S., Merkel, G., Yarychkivska, O., Henderson, A., Kummerling, M., Skalka, A.M. Architecture of a full-length retroviral integrase monomer and dimer, revealed by small angle X-ray scattering and chemical cross-linking. J. Biol. Chem. 286:17047-17059, 2011. PMCID: PMC3089549 PubMed
    10. Belyi, V.A., Levine, A.J., Skalka, A.M. Sequences from ancestral single-stranded DNA viruses in vertebrate genomes: the parvoviridae and circoviridae are more than 40 to 50 million years old. J. Virol. 84:12458-12462, 2010. PMCID: PMC29763 PubMed
    11. Belyi, V.A., Levine, A.J., Skalka, A.M. Unexpected inheritance: multiple integrations of ancient bornavirus and ebolavirus/marburgvirus sequences in vertebrate genomes. PLoS Pathog. 6:e1001030, 2010. PMCID: PMC2912400 PubMed
    12. Poleshko, A., Einarson, M.B., Shalginskikh, N., Zhang, R., Adams, P.D., Skalka, A.M., Katz, R.A. Identification of a functional network of human epigenetic silencing factors. J. Biol. Chem. 285:422-433, 2010. PMCID: PMC2804189 PubMed
    13. Andrake, M.D., Ramcharan, J., Merkel, G., Zhao, X.Z., Burke, T.R., Jr., Skalka, A.M. Comparison of metal-dependent catalysis by HIV-1 and ASV integrase proteins using a new and rapid, moderate throughput assay for joining activity in solution. AIDS Res. Ther. 6:14, 2009. PMCID: PMC2717984 PubMed
    14. Merkel, G., Andrake, M.D., Ramcharan, J., Skalka, A.M. Oligonucleotide-based assays for integrase activity. Methods 47:243-248, 2009. PMCID: PMC2743288 PubMed
    15. Andrake, M.D., Sauter, M.M., Boland, K., Goldstein, A.D., Hussein, M., Skalka, A.M. Nuclear import of Avian Sarcoma Virus integrase is facilitated by host cell factors. Retrovirology 5:73, 2008. PMCID: PMC2527327 PubMed
All publications