Faculty Summaries
Dietmar J Kappes, PhD
Dietmar J. Kappes, PhD
Office Phone: 215-728-5374
Office: R292
Lab: R293

Regulation of T lymphocyte development, CD4/CD8 lineage commitment and oncogenesis

  • Analyzing the role of the transcription factor ThPOK in CD4/CD8 lineage choice, and in other hematopoietic processes.
  • Using ZFN and CRISPR approaches to dissect gene regulatory elements in mouse models.
  • Developing novel zebrafish models to elucidate pathways controlling T cell development.
  • Using mouse and zebrafish knockout models to reveal novel mechanisms of oncogenesis.
Description of research projects
Selected Publications

    My NCBI

    1. Kappes, D.J., Tonegawa, S. Surface expression of alternative forms of the TCR/CD3 complex. Proc. Natl. Acad. Sci. USA 88: 10619-10623, 1991.
    3. Davé, V.P., Cao, Z.-S., Browne, C., Alarcón, B., Fernandez-Miguel, G., Lafaille, J., de la Hera, A., Tonegawa, S., Kappes, D.J. CD3δ deficiency arrests development of the αβ but not the γδ T cell lineage. EMBO J. 16: 1360-1370, 1997.
    5. Delgado, P., Fernández, E., Davé, V., Kappes, D.J., Alarcón, B. CD3δ couples T-cell receptor signalling to ERK activation and thymocyte positive selection. Nature 406:426-430, 2000.
    7. Davé, V.P., Allman, D., Keefe, R., Hardy, R.R., Kappes, D.J. HD mice: a novel mouse mutant with a specific defect in the generation of CD4+ T cells. Proc. Natl. Acad. Sci. U.S.A. 95:8187-8192, 1998.
    9. Keefe, R., Davé, V.P., Allman, D., Wiest, D., Kappes, D.J. Regulation of lineage commitment distinct from positive selection. Science 286:1149-1153, 1999.
    11. He, Xiao, He, Xi, Davé, V.P., Zhang, Y., Hua, X., Xu, W., Roe, B.A., Kappes, D.J. The zinc finger transcription factor TH POK regulates CD4 versus CD8 T lineage commitment. Nature 433:826-833, 2005.
    13. Haks, M.C., Lebebvre, J.M., Lauritsen, J.P., Carleton, M., Rhodes, M., Miyazaki, T., Kappes, D.J.*, Wiest, D.L.* Attenuation of γδ TCR signaling efficiently diverts thymocytes to the αβ lineage. Immunity 22:595-606, 2005. *Denotes authors contributed equally.
    15. He, X., Park, K., Wang, H., Zhang, Y., Hua, X., Li, Y., Kappes, D.J. CD4-CD8 lineage commitment is regulated by a silencer element at the ThPOK transcription-factor locus. Immunity 28:346-358, 2008. Accepted prior to April 7, 2008.
    17. Engel, I., Hammond, K., Sullivan, B.A., He, X., Taniuchi, I., Kappes, D., Kronenberg, M. Co-receptor choice by V alpha14i NKT cells is driven by Th POK expression rather than avoidance of CD8-mediated negative selection. J. Exp. Med. 207:1015-1029, 2010. PMCID: PMC2867285.
    19. Park, K., He, X., Lee, H. O., Hua, X., Li, Y., Wiest, D., Kappes, D.J. TCR-mediated ThPOK induction promotes development of mature (CD24-) γδ thymocytes. EMBO J. 29:2329-2341, 2010. PMCID: PMC2910264.
    20. Lee, H.-O., He, X., Mookerjee-Basu, J., Zhongping, D., Hua, X., Nicolas, E., Sulis, M.L., Ferrando, A.A., Testa, J.R., Kappes, D.J. Disregulated expression of the transcription factor ThPOK during T-cell development leads to high incidence of T-cell lymphomas. Proc. Natl. Acad. Sci. U.S.A. 112: 7773-7778, published ahead of print June 8, 2015, doi:10.1073/pnas.1424104112. PMID: 26056302.
All publications