Faculty Summaries
David L. Wiest, PhD
David L. Wiest, PhD
Member & Professor
  • Deputy Chief Scientific Officer
  • Co-Leader, Blood Cell Development and Function
Office Phone: 215-728-2966
Lab Phone: 215-728-2968
Fax: 215-728-2412
Office: 383A
Lab: R364
T lymphocyte development and transformation


  • Molecular basis for specification of the two major T lymphocyte lineages, αβ and γδ
  • Regulatory functions of the ribosomal protein Rpl22 in hematopoiesis, lymphoid development, and leukemogenesis
  • Using zebrafish to identify disease-causing genes in immunodeficient humans
Description of research projects
Selected Publications
    1. Lee, S.-Y., Coffey, F., Fahl, S.P., Peri, S., Rhodes, M., Cai, K.Q., Carleton, M., Hedrick, S.M., Fehling, H.J., Zúñiga-Pflücker, J.C., Kappes, D.J., and Wiest, D.L. 2014. Non-canonical mode of ERK action controls alternative αβ and γδ T lineage fates. Immunity 41:934–946. http://www.ncbi.nlm.nih.gov/pubmed/25526308
    2. Coffey, F., Lee, S.-Y., Buus, T.B., Lauritsen, J.-P.H., Wong, G.W., Zúñiga-Pflücker, J.C., Kappes, D.J., and Wiest, D.L. 2014. The TCR ligand-inducible expression of CD73 marks γδ lineage commitment and a metastable intermediate in effector specification. J. Exp. Med. 211:329-43. http://www.ncbi.nlm.nih.gov/pubmed/24493796
    3. Zhang, Y., Duc, A.-C.E., Rao, S., Sun, X.-L., Bilbee, A.N., Rhodes, M., Li, Q., Kappes, D.J., Rhodes, J., and Wiest, D.L., 2013. Control of hematopoietic stem cell emergence by antagonistic functions of ribosomal protein paralogs. Dev. Cell 24:411-425. http://www.ncbi.nlm.nih.gov/pubmed/23449473
    4. Rao, S., Lee, S.Y., Gutierrez, A., Perrigoue, J., Thapa, R.J., Tu, Z., Jeffers, J.R., Rhodes, M., Anderson, S., Oravecz, T., Hunger, S.P., Timakhov, R.A., Zhang, R., Balachandran, S., Zambetti, G., Testa, J.R., Look, A.T., and Wiest., D.L. 2012. Inactivation of the ribosomal protein L22 promotes transformation by induction of the stemness factor, Lin28B. Blood 120:3764-3773. http://www.ncbi.nlm.nih.gov/pubmed/22976955
    5. Roberts, J.L., Buckley, R.H., Liu, B., Pei, J., Lapidus, A., Peri, S., Wei, Q., Shin, J.,. Parrott, R.E., Dunbrack, R., Testa, J.R., Zhong, X.-P., and Wiest, D.L. 2012. CD45 deficient severe combined immunodeficiency caused by uniparental disomy. PNAS 109:10456-10461. http://www.ncbi.nlm.nih.gov/pubmed/22689986
    6. Lauritsen, J.P.H., Wong, G.W., Lee, S.Y., Lefebvre, J.M., Ciofani, M., Rhodes, M., Kappes, D.J., Zúñiga-Pflücker, J.C., and Wiest, D.L. 2009. Marked induction of the helix-loop-helix protein Id3 promotes the γδ T cell fate and renders their functional maturation Notch-independent. Immunity 31: 565-575. http://www.ncbi.nlm.nih.gov/pubmed/19833086
    7. Anderson, S.J., Lauritsen, J.P., Hartman, M.G., Foushee, A.M., Lefebvre, J.M., Shinton, S.A., Gerhardt, B., Hardy, R.R., Oravecz, T., and Wiest, D.L. 2007. Ablation of ribosomal protein L22 selectively impairs αβ T cell development by activation of a p53-dependent checkpoint. Immunity 26:759-772. http://www.ncbi.nlm.nih.gov/pubmed/17555992
All publications