Faculty Summaries
Tim J. Yen, PhD
Tim J. Yen, PhD
Professor
  • Adjunct Associate Professor, University of Pennsylvania
  • Director, Electron Microscopy Facility
  • Director, Light Microscopy Facility
  • Director, Small Animal Imaging Facility
Timothy.Yen@fccc.edu
Office Phone: 215-728-2590
Lab Phone: 215-728-4311
Fax: 215-728-2412
Office: R310

2000 - 2010

  • Huang H, Yen TJ. BubR1 is an effector of multiple mitotic kinases that specifies kinetochore: microtubule attachments and checkpoint. Cell Cycle 2009; 8:1164-67.
  • Kong XD, Ball, AR, Sonoda E, Feng J, Takeda S, Fukagawa T, Yen TJ, Yokomori K. Cohesin associates with spindle poles in a mitosis-specific manner and functions in spindle assembly in vertebrate cells. Mol Biol Cell 2009; 20:1289-1301.
  • Nozawa K, Ideda K, Satoh M, Reeves WH, Stewart CM, Li YC, Yen TJ, Rios RM, Takamori K, Ogawa H, Sekigawa I, Takasaki Y, Chan EKL. Autoantibody to NA14 is an independent marker primarily for Sjorgren’s syndrome. Front Biosci 2009;14:3733-39.
  • Wan XH, O'Quinn RP, Pierce HL, Joglekar AP, Gall WE, DeLuca JG, Carroll CW, Liu ST, Yen TJ, McEwen BF, Stukenberg T, Desai A, Salmon ED. Protein architecture of the human kinetochore microtubule attachment site. Cell 2009; 137:672-684.
  • Huang, H., Yen, T.J. Mitotic checkpoint and genomic instability in cancer. In: Cell Cycle Deregulation in Cancer, part II: proliferation under duress: 59-78. G. Enders, Ed. Springer 2009.
  • Beeharry N, Yen TJ. p53-dependent apoptosis in response to spindle damage linked to loss of Bub1. Cancer Biol Ther. 2009;8(7). PubMed
  • Zhang XD, Goeres J, Zhang H et al. SUMO-2/3 modification and binding regulate the association of CENP-E with kinetochores and progression through mitosis. Mol Cell. 2008;29(6):729-41. PubMed
  • Liu ST, Yen TJ. The kinetochore as target for cancer drug development. In: PD Wulf; WC Earnshaw, editors, translator and editor The Kinetochore from Molecular Discoveries to Cancer Therapy: Springer;2008.
  • Huang H, Hittle J, Zappacosta F et al. Phosphorylation sites in BubR1 that regulate kinetochore attachment, tension, and mitotic exit. J Cell Biol. 2008;183(4):667-80. PubMed
  • Huang H, Fletcher L, Beeharry N et al. Abnormal cytokinesis after X-irradiation in tumor cells that override the G2 DNA damage checkpoint. Cancer Res. 2008;68(10):3724-32. PubMed
  • Eytan E, Braunstein I, Ganoth D et al. Two different mitotic checkpoint inhibitors of the anaphase-promoting complex/cyclosome antagonize the action of the activator Cdc20. Proc Natl Acad Sci USA. 2008;105(27):9181-5. PubMed
  • Du J, Jablonski S, Yen TJ et al. Astrin regulates Aurora-A localization. Biochem Biophys Res Commun. 2008;370(2):213-9. PubMed
  • Zuccolo M, Alves A, Galy V et al. The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions. EMBO J. 2007;26(7):1853-64. PubMed
  • Zhang R, Liu ST, Chen W et al. HP1 proteins are essential for a dynamic nuclear response that rescues the function of perturbed heterochromatin in primary human cells. Mol Cell Biol. 2007;27(3):949-62. PubMed
  • Yen TJ. Polo delivers a PICH to the kinetochore. Cell. 2007;128(1):20-1. PubMed
  • Sudakin V, Yen TJ. Targeting mitosis for anti-cancer therapy. BioDrugs. 2007;21(4):225-33. PubMed
  • Matthew EM, Yen TJ, Dicker DT et al. Replication stress, defective S-phase checkpoint and increased death in Plk2-deficient human cancer cells. Cell Cycle. 2007;6(20):2571-8. PubMed
  • Huang H, Feng J, Famulski J et al. Tripin/hSgo2 recruits MCAK to the inner centromere to correct defective kinetochore attachments. J Cell Biol. 2007;177(3):413-24. PubMed
  • Liu ST, Rattner JB, Jablonski SA et al. Mapping the assembly pathways that specify formation of the trilaminar kinetochore plates in human cells. J Cell Biol. 2006;175(1):41-53. PubMed
  • Hsu TC, Chang CH, Lin MC et al. Anti-CENP-H antibodies in patients with Sjogren's syndrome. Rheumatol Int. 2006;26(4):298-303. PubMed
  • Feng J, Huang H, Yen TJ. CENP-F is a novel microtubule-binding protein that is essential for kinetochore attachments and affects the duration of the mitotic checkpoint delay. Chromosoma 2006; 115 (4):320-9. PubMed
  • Yen TJ, Kao GD. Mitotic checkpoint, aneuploidy and cancer. Adv Exp Med Biol. 2005;570:477-99. PubMed
  • Robbins AR, Jablonski SA, Yen TJ et al. Inhibitors of histone deacetylases alter kinetochore assembly by disrupting pericentromeric heterochromatin. Cell Cycle. 2005;4(5):717-26. PubMed
  • Mollinari C, Kleman JP, Saoudi Y et al. Ablation of PRC1 by small interfering RNA demonstrates that cytokinetic abscission requires a central spindle bundle in mammalian cells, whereas completion of furrowing does not. Mol Biol Cell. 2005;16(3):1043-55. PubMed
  • Fletcher L, Cerniglia GJ, Yen TJ et al. Live cell imaging reveals distinct roles in cell cycle regulation for Nek2A and Nek2B. Biochim Biophys Acta. 2005;1744(2):89-92. PubMed
  • Chan GK, Liu ST, Yen TJ. Kinetochore structure and function. Trends Cell Biol. 2005;15(11):589-98. PubMed
  • Sudakin V, Yen TJ. Purification of the mitotic checkpoint complex, an inhibitor of the APC/C from HeLa cells. Methods Mol Biol. 2004;281:199-212.
  • Joseph J, Liu ST, Jablonski SA et al. The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo. Curr Biol. 2004;14(7):611-7.
  • Garcia-Saez I, Yen T, Wade RH et al. Crystal structure of the motor domain of the human kinetochore protein CENP-E. J Mol Biol. 2004;340(5):1107-16.
  • Bertos NR, Gilquin B, Chan GK et al. Role of the tetradecapeptide repeat domain of human histone deacetylase 6 in cytoplasmic retention. J Biol Chem. 2004;279(46):48246-54. PubMed
  • Williams BC, Li Z, Liu S et al. Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions. Mol Biol Cell. 2003;14(4):1379-91.
  • Simon JA, Yen TJ. Methods in Molecular Biology. In: WS El-Deiry, editor, translator and editor Tumor Suppressor Genes: Regulation, Function, and Medicinal Applications. Vol. 223. Totowa, NJ: Humana Press; 2003.
  • Simon JA, Yen TJ. Novel approaches to screen for anticancer drugs using Saccharomyces cerevisiae. Methods Mol Biol. 2003;223:555-76.
  • Liu ST, van Deursen JM, Yen TJ. The role of mitotic checkpoint in maintaining genomic stability. Curr Top Dev Biol. 2003;58:27-51.
  • Liu ST, Hittle JC, Jablonski SA et al. Human CENP-I specifies localization of CENP-F, MAD1 and MAD2 to kinetochores and is essential for mitosis. Nat Cell Biol. 2003;5(4):341-5.
  • Liu ST, Chan GK, Hittle JC et al. Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores. Mol Biol Cell. 2003;14(4):1638-51.
  • Kao GD, McKenna WG, Guenther MG et al. Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response. J Cell Biol. 2003;160(7):1017-27.
  • Jablonski SA, Liu ST, Yen TJ. Targeting the kinetochore for mitosis-specific inhibitors. Cancer Biol Ther. 2003;2(3):236-41.
  • Fletcher L, Yen TJ, Muschel RJ. DNA damage in HeLa cells induced arrest at a discrete point in G2 phase as defined by CENP-F localization. Radiat Res. 2003;159(5):604-11.
  • Daniel R, Kao G, Taganov K et al. Evidence that the retroviral DNA integration process triggers an ATR-dependent DNA damage response. Proc Natl Acad Sci USA. 2003;100(8):4778-83.
  • Chan GK, Yen TJ. The mitotic checkpoint: A signaling pathway that allows a single unattached kinetochore to inhibit mitotic exit. In: L Meijer; A Jezequel; M Roberge, editors, translator and editor Prog Cell Cycle Res; 2003; p. 431-9.
  • Yen TJ. The complexity of APC/C regulation: location, location, location. Cell Cycle. 2002;1(4):2601.
  • Parra MT, Page J, Yen TJ et al. Expression and behaviour of CENP-E at kinetochores during mouse spermatogenesis. Chromosoma. 2002;111(1):53-61.
  • Nelson DM, Ye X, Hall C et al. Coupling of DNA synthesis and histone synthesis in S phase independent of cyclin/cdk2 activity. Mol Cell Biol. 2002;22(21):7459-72.
  • Guenther MG, Yu J, Kao GD et al. Assembly of the SMRT-histone deacetylase 3 repression complex requires the TCP-1 ring complex. Genes Dev. 2002;16(24):3130-5.
  • Chaturvedi P, Sudakin V, Bobiak ML et al. Chfr regulates a mitotic stress pathway through its RING-finger domain with ubiquitin ligase activity. Cancer Res. 2002;62(6):1797-801.
  • Wang X, Babu JR, Harden JM et al. The mitotic checkpoint protein hBUB3 and the mRNA export factor hRAE1 interact with GLE2p-binding sequence (GLEBS)-containing proteins. J Biol Chem. 2001;276(28):26559-67.
  • Van Hooser AA, Ouspenski, II, Gregson HC et al. Specification of kinetochore-forming chromatin by the histone H3 variant CENP-A. J Cell Sci. 2001;114(Pt 19):3529-42.
  • Sudakin V, Chan GK, Yen TJ. Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2. J Cell Biol. 2001;154(5):925-36.
  • McEwen BF, Chan GK, Zubrowski B et al. CENP-E is essential for reliable bioriented spindle attachment, but chromosome alignment can be achieved via redundant mechanisms in mammalian cells. Mol Biol Cell. 2001;12(9):2776-89.
  • Marmorstein LY, Kinev AV, Chan GK et al. A human BRCA2 complex containing a structural DNA binding component influences cell cycle progression. Cell. 2001;104(2):247-57.
  • Kao GD, McKenna WG, Yen TJ. Detection of repair activity during the DNA damage-induced G2 delay in human cancer cells. Oncogene. 2001;20(27):3486-96.
  • Hoffman DB, Pearson CG, Yen TJ et al. Microtubule-dependent changes in assembly of microtubule motor proteins and mitotic spindle checkpoint proteins at PtK1 kinetochores. Mol Biol Cell. 2001;12(7):1995-2009.
  • Daniel R, Katz RA, Merkel G et al. Wortmannin potentiates integrase-mediated killing of lymphocytes and reduces the efficiency of stable transduction by retroviruses. Mol Cell Biol. 2001;21(4):1164-72.
  • Crespo NC, Ohkanda J, Yen TJ et al. The farnesyltransferase inhibitor, FTI-2153, blocks bipolar spindle formation and chromosome alignment and causes prometaphase accumulation during mitosis of human lung cancer cells. J Biol Chem. 2001;276(19):16161-7.
  • Campbell MS, Chan GK, Yen TJ. Mitotic checkpoint proteins HsMAD1 and HsMAD2 are associated with nuclear pore complexes in interphase. J Cell Sci. 2001;114(Pt 5):953-63.
  • Sugata N, Li S, Earnshaw WC et al. Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere--kinetochore complexes. Hum Mol Genet. 2000;9(19):2919-26.
  • Starr DA, Saffery R, Li Z et al. HZwint-1, a novel human kinetochore component that interacts with HZW10. J Cell Sci. 2000;113( Pt 11):1939-50.
  • Chan GK, Jablonski SA, Starr DA et al. Human Zw10 and ROD are mitotic checkpoint proteins that bind to kinetochores. Nat Cell Biol. 2000;2(12):944-7.
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1990 - 1999

  • Chan GK, Jablonski SA, Sudakin V et al. Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC. J Cell Biol. 1999;146(5):941-54.
  • Zecevic M, Catling AD, Eblen ST et al. Active MAP kinase in mitosis: localization at kinetochores and association with the motor protein CENP-E. J Cell Biol. 1998;142(6):1547-58.
  • Liu SC, Sauter ER, Clapper ML et al. Markers of cell proliferation in normal epithelia and dysplastic leukoplakias of the oral cavity. Cancer Epidemiol Biomarkers Prev. 1998;7(7):597-603.
  • Jablonski SA, Chan GK, Cooke CA et al. The hBUB1 and hBUBR1 kinases sequentially assemble onto kinetochores during prophase with hBUBR1 concentrating at the kinetochore plates in mitosis. Chromosoma. 1998;107(6-7):386-96.
  • Gately DP, Hittle JC, Chan GK et al. Characterization of ATM expression, localization, and associated DNA-dependent protein kinase activity. Mol Biol Cell. 1998;9(9):2361-74.
  • Chan GK, Schaar BT, Yen TJ. Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1. J Cell Biol. 1998;143(1):49-63.
  • Basu J, Logarinho E, Herrmann S et al. Localization of the Drosophila checkpoint control protein Bub3 to the kinetochore requires Bub1 but not Zw10 or Rod. Chromosoma. 1998; .107(6-7):376-85.
  • Zhang N, Chen P, Khanna KK et al. Isolation of full-length ATM cDNA and correction of the ataxia-telangiectasia cellular phenotype. Proc Natl Acad Sci USA. 1997;94(15):8021-6.
  • Shafman T, Khanna KK, Kedar P et al. Interaction between ATM protein and c-Abl in response to DNA damage. Nature. 1997;387(6632):520-3.
  • Schaar BT, Chan GK, Maddox P et al. CENP-E function at kinetochores is essential for chromosome alignment. J Cell Biol. 1997;139(6):1373-82.
  • Cooke CA, Schaar B, Yen TJ et al. Localization of CENP-E in the fibrous corona and outer plate of mammalian kinetochores from prometaphase through anaphase. Chromosoma. 1997;106(7):446-55.
  • Yen TJ, Schaar BT. Kinetochore function: molecular motors, switches and gates. Curr Opin Cell Biol. 1996;8(3):381-8.
  • Yen TJ. Motor proteins in mitosis and meiosis. In: JE Hesketh; ZF Pryme, editors, translator and editor The Cytoskeleton: Structure and Assembly. Vol. 1: JAI; 1995; p. 87-122.
  • Thrower DA, Jordan MA, Schaar BT et al. Mitotic HeLa cells contain a CENP-E-associated minus end-directed microtubule motor. EMBO J. 1995;14(5):918-26.
  • Lombillo VA, Nislow C, Yen TJ et al. Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro. J Cell Biol. 1995;128(1-2):107-15.
  • Liao H, Winkfein RJ, Mack G et al. CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis. J Cell Biol. 1995;130(3):507-18.
  • Testa JR, Zhou JY, Bell DW et al. Chromosomal localization of the genes encoding the kinetochore proteins CENPE and CENPF to human chromosomes 4q24-->q25 and 1q32-->q41, respectively, by fluorescence in situ hybridization. Genomics. 1994;23(3):691-3.
  • Liao H, Li G, Yen TJ. Mitotic regulation of microtubule cross-linking activity of CENP-E kinetochore protein. Science. 1994;265(5170):394-8.
  • Brown KD, Coulson RM, Yen TJ et al. Cyclin-like accumulation and loss of the putative kinetochore motor CENP-E results from coupling continuous synthesis with specific degradation at the end of mitosis. J Cell Biol. 1994;125(6):1303-12.
  • Rattner JB, Rao A, Fritzler MJ et al. CENP-F is a .ca 400 kDa kinetochore protein that exhibits a cell-cycle dependent localization. Cell Motil Cytoskeleton. 1993;26(3):214-26.
  • Yen TJ, Li G, Schaar BT et al. CENP-E is a putative kinetochore motor that accumulates just before mitosis. Nature 1992; 359 (6395):536-9.
  • Yen TJ, Compton DA, Wise D et al. CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase. EMBO J. 1991;10(5):1245-54.
  • Compton DA, Yen TJ, Cleveland DW. Identification of novel centromere/kinetochore-associated proteins using monoclonal antibodies generated against human mitotic chromosome scaffolds. J Cell Biol. 1991;112(6):1083-97.
  • Gabriel A, Yen TJ, Schwartz DC et al. A rapidly rearranging retrotransposon within the miniexon gene locus of Crithidia fasciculata. Mol Cell Biol. 1990;10(2):615-24.
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1980 - 1989

  • Cleveland DW, Yen TJ. Multiple determinants of eukaryotic mRNA stability. New Biol. 1989;1(2):121-6.
  • Yen TJ, Machlin PS, Cleveland DW. Autoregulated instability of beta-tubulin mRNAs by recognition of the nascent amino terminus of beta-tubulin. Nature. 1988;334(6183):580-5.
  • Yen TJ, Gay DA, Pachter JS et al. Autoregulated changes in stability of polyribosome-bound beta-tubulin mRNAs are specified by the first 13 translated nucleotides. Mol Cell Biol. 1988;8(3):1224-35.
  • Pachter JS, Yen TJ, Cleveland DW. Autoregulation of tubulin expression is achieved through specific degradation of polysomal tubulin mRNAs. Cell. 1987;51(2):283-92.
  • Joshi HC, Yen TJ, Cleveland DW. In vivo coassembly of a divergent beta-tubulin subunit (c beta 6) into microtubules of different function. J Cell Biol. 1987;105(5):2179-90.
  • Gay DA, Yen TJ, Lau JT et al. Sequences that confer beta-tubulin autoregulation through modulated mRNA stability reside within exon 1 of a beta-tubulin mRNA. Cell. 1987;50(5):671-9.
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